Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Search research articles

Related Experiment Videos

Methods for evaluating topical antibacterial agents on human skin.

R R Marples, A M Kligman

Antimicrobial Agents and Chemotherapy

|March 1, 1974

Summary

This study presents three methods to evaluate antibacterial agents for controlling skin microorganisms. The research assessed their ability to prevent, suppress, and maintain antibacterial effects on human skin flora.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Assessment of the atrophogenicity of corticosteroids to human skin using non-invasive methods.

Skin research and technology : official journal of International Society for Bioengineering and the Skin (ISBS) [and] International Society for Digital Imaging of Skin (ISDIS) [and] International Society for Skin Imaging (ISSI)·2016

Same author

The future of bioengineering A dermatologist's perspective.

Skin research and technology : official journal of International Society for Bioengineering and the Skin (ISBS) [and] International Society for Digital Imaging of Skin (ISDIS) [and] International Society for Skin Imaging (ISSI)·2016

Same author

Mechanisms of eccrine anidrosis. I. High level blockade.

The Journal of investigative dermatology·2015

Same author

The SLS provocative patch test in allergic contact sensitization.

The Journal of investigative dermatology·2015

Same author

Corneobiology and corneotherapy--a final chapter.

International journal of cosmetic science·2011

Same author

A new species of Cephalosporium in Madura foot (Cephalosporium granulomatis).

Journal of bacteriology·2010

Area of Science:

Microbiology
Dermatology
Antimicrobial Agents

Background:

Human skin harbors a complex microflora.
Occlusive conditions can lead to rapid microbial overgrowth.
Assessing antibacterial efficacy on skin is crucial for hygiene and infection control.

Purpose of the Study:

To present and validate three distinct procedures for evaluating antibacterial chemicals and formulations.
To quantify the impact of these agents on skin microflora under occlusive conditions.
To assess the duration of antibacterial effect post-application.

Main Methods:

Development of three standardized procedures for antibacterial skin testing.
Quantification of resident skin microflora.
Application of occlusive dressings to simulate conditions promoting microbial growth.
Evaluation of antibacterial activity at different stages: prevention, suppression, and persistence.

Main Results:

The presented procedures effectively appraised the efficacy of antibacterial agents.
Data demonstrated the capacity of tested agents to prevent microflora expansion.
Suppression of existing microflora and assessment of 3-day persistent antibacterial effect were achieved.

Conclusions:

The developed methods provide a reliable framework for assessing antibacterial efficacy on human skin.
These procedures are valuable for the development and validation of new antimicrobial products.
The study highlights the importance of evaluating both immediate and persistent effects of antibacterial treatments.

Related Experiment Videos