Cryosurgical devitalization of bone and its regenreation; An experimental study with animals

In an experimental study using rabbits, bone is supercooled by means of liquid nitrogen flowing through a silicone chamber. The surrounding soft tissue is protected by a heating element of silicone. The histological findings in the stages of devitalization of osteocytes are described. X-rays and fluorescent labelling were used, searching for the time of total recalcification in supercooled bone.