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Related Experiment Videos

Structure and function in urinary bladder of foetal sheep.

V M France, M W Stanier, F B Wooding

    The Journal of Physiology
    |June 1, 1974
    PubMed
    Summary

    Sheep fetal bladder epithelium develops fully by 50 days. Tight junctions mature early, preventing lanthanum penetration by 90 days, while antidiuretic hormone affects sodium but not water transport.

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    Archives of disease in childhood·2010

    Area of Science:

    • Urothelial biology
    • Fetal development
    • Epithelial transport

    Background:

    • The urinary bladder epithelium's structure and function are crucial for waste excretion and maintaining homeostasis.
    • Understanding fetal bladder development provides insights into congenital urinary tract abnormalities.

    Purpose of the Study:

    • To investigate the developmental changes in sheep fetal urinary bladder epithelium structure and permeability.
    • To assess the role of tight junctions and the effect of antidiuretic hormone (ADH) on ion and water transport.

    Main Methods:

    • Electron microscopy was used to examine epithelial cell structure at various gestational ages (50-141 days).
    • In vitro permeability studies measured unidirectional fluxes of sodium and water across the bladder wall.
    • Lanthanum chloride (LaCl3) was used to assess tight junction integrity.
    • The effects of ADH on water and sodium transport were evaluated.

    Main Results:

    • The luminal plasmalemma was fully developed by 50 days gestation.
    • Tight junctions were functional by 90 days, effectively blocking lanthanum.
    • No net water movement was observed, but sodium efflux exceeded influx.
    • ADH did not affect water transport but reduced sodium efflux, altering the flux ratio.

    Conclusions:

    • Sheep fetal bladder epithelium exhibits mature surface structure early in development.
    • Tight junctions mature early and remain a barrier, even when ADH causes intercellular space swelling.
    • ADH modulates sodium transport in the fetal bladder, suggesting a role in regulating electrolyte balance before birth.

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