Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Polycyclic aromatic isothiocyanate compounds as fluorescent labeling reagents.

J E Sinsheimer, V Jagodić, L J Polak

    Journal of Pharmaceutical Sciences
    |June 1, 1975
    PubMed
    Summary

    New fluorescent polycyclic aromatic isothiocyanates were synthesized for protein labeling and microanalysis. Acridine derivatives showed the most promise as novel fluorescent agents.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Organic waste as a sustainable feedstock for platform chemicals.

    Faraday discussions·2017
    Same author

    Aminoalkylphenols as antimalarials; simple substituted alpha-aminocresols.

    Journal of the American Chemical Society·2010
    Same author

    Adrenal proteins bound by a reactive intermediate of mitotane.

    Cancer chemotherapy and pharmacology·1997
    Same author

    Gas chromatographic-electron capture determination of 2,4'-dichlorodiphenylacetic acid from in-vitro adrenal transformations of mitotane and its analogs.

    Journal of pharmaceutical and biomedical analysis·1996
    Same author

    Bovine adrenal cortex transformations of mitotane [1-(2-chlorophenyl)-1-(4-chlorophenyl)-2,2-dichloroethane; o,p'-DDD] and its p,p'- and m,p'-isomers.

    Biochemical pharmacology·1995
    Same author

    Metabolic activation and binding of mitotane in adrenal cortex homogenates.

    Journal of pharmaceutical sciences·1995

    Area of Science:

    • Organic Chemistry
    • Analytical Chemistry
    • Biochemistry

    Background:

    • Fluorescent labeling is crucial for protein analysis and microanalytical techniques.
    • Development of novel fluorescent agents is an ongoing area of research.
    • Polycyclic aromatic compounds offer a scaffold for designing new fluorescent molecules.

    Purpose of the Study:

    • To synthesize novel polycyclic aromatic isothiocyanates.
    • To evaluate their potential as fluorescent agents for protein labeling.
    • To explore their utility in microanalytical techniques.

    Main Methods:

    • Synthesis of various polycyclic aromatic isothiocyanates.
    • Spectroscopic analysis to determine fluorescence properties.
    • Evaluation of fluorescence intensity and stability.

    Related Experiment Videos

    Main Results:

    • Several polycyclic aromatic isothiocyanates were successfully synthesized.
    • Relative fluorescence of synthesized compounds, intermediates, and derivatives were established.
    • 9-Acridine derivatives exhibited the highest fluorescence among the tested compounds.

    Conclusions:

    • Polycyclic aromatic isothiocyanates are viable candidates for fluorescent agent development.
    • 9-Acridine derivatives show significant potential for protein labeling and microanalysis.
    • Further research into acridine-based fluorescent probes is warranted.