Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

An electron-microscope study of beta-glucuronidase crystals.

M R Dickson, M Stewart, D E Hawley

    The Biochemical Journal
    |July 1, 1979
    PubMed
    Summary
    This summary is machine-generated.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Segmented filamentous bacteria in the rodent small intestine: Their colonization of growing animals and possible role in host resistance toSalmonella.

    Microbial ecology·2013
    Same author

    Negative staining of freshwater bacterioneuston sampled directly with electron microscope specimen support grids.

    Microbial ecology·2013
    Same author

    Association studies of transforming growth factor-beta 1 and Alzheimer's disease.

    American journal of medical genetics. Part B, Neuropsychiatric genetics : the official publication of the International Society of Psychiatric Genetics·2005
    Same author

    Drosophila E2f2 promotes the conversion from genomic DNA replication to gene amplification in ovarian follicle cells.

    Development (Cambridge, England)·2001
    Same author

    Identification and modification of the uridine-binding site of the UDP-GalNAc (GlcNAc) pyrophosphorylase.

    The Journal of biological chemistry·2000
    Same author

    Place of birth and shoulder dystocia.

    British journal of obstetrics and gynaecology·1999

    Crystallized beta-glucuronidase from rat preputial glands revealed a p6 symmetry. Analysis suggests a tetrahedral arrangement of its four subunits, offering insights into enzyme structure.

    Area of Science:

    • Biochemistry
    • Structural Biology
    • Enzymology

    Background:

    • Beta-glucuronidase is a key enzyme involved in various biological processes.
    • Understanding the quaternary structure of beta-glucuronidase is crucial for elucidating its function.

    Purpose of the Study:

    • To determine the three-dimensional structure of beta-glucuronidase from rat preputial glands.
    • To investigate the arrangement of subunits within the beta-glucuronidase molecule.

    Main Methods:

    • Crystallization of beta-glucuronidase.
    • Image processing using Fourier methods.
    • Analysis of projected symmetry and molecular outline.

    Main Results:

    • Beta-glucuronidase was crystallized into thin sheets with p6 symmetry in projection (a = 20.2 nm).

    Related Experiment Videos

  • Filtered images achieved a resolution of 2.2 nm.
  • The molecular projection exhibited an approximately triangular outline.

    • Conclusions:

    • The observed triangular projection suggests a tetrahedral arrangement of the four subunits.
    • This finding provides a structural model for the beta-glucuronidase enzyme.