Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Ion transport in the simplest single file pore.

B W Urban, S B Hladky

    Biochimica Et Biophysica Acta
    |July 5, 1979
    PubMed
    Summary

    This study presents a kinetic model for single-file ion transport through pores, detailing how ion interactions influence flux. The model predicts phenomena like ion blocking and rectification, crucial for understanding biological transport mechanisms.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Fast and slow interactions of n-alkanols with human 5-HT3A receptors: Implications for anesthetic mechanisms.

    Biochimica et biophysica acta·2015
    Same author

    Direct effects of morphine but not of fentanyl-type opioids on human 5-HT3A receptors in outside-out patch-clamp studies.

    European journal of pain (London, England)·2014
    Same author

    Which agonist properties are important for the activation of 5-HT3A receptors?

    Biochimica et biophysica acta·2013
    Same author

    The site of anesthetic action.

    Handbook of experimental pharmacology·2008
    Same author

    The effects of morphine on human 5-HT3A receptors.

    Anesthesia and analgesia·2006
    Same author

    Correlation of the A-Line ARX index with acoustically evoked potential amplitude.

    British journal of anaesthesia·2006

    Area of Science:

    • Biophysics
    • Physical Chemistry
    • Computational Biology

    Background:

    • Single-file ion transport is fundamental to biological processes.
    • Understanding ion movement through pores requires detailed kinetic models.
    • Gramicidin A channels serve as a model system for studying ion transport.

    Purpose of the Study:

    • To develop a kinetic scheme for single-file transport of up to two ions of different species through pores.
    • To derive flux solutions without assuming symmetry or voltage/activity dependence of rate constants.
    • To explore specific assumptions for pore occupancy and ion transfer rates.

    Main Methods:

    • Development of a kinetic scheme for ion transport.
    • Derivation of flux equations based on rate constants for entry, exit, and transfer.
    • Analysis of pore behavior under specific assumptions regarding ion occupancy and transfer speed.

    Main Results:

    • The model predicts distinct linear regions for conductance-activity relationships in symmetrical pores.
    • Zero current potentials are dependent on absolute ion activities and their ratios.
    • Specific assumptions lead to predictions of flux ratio exponents near 2, ion blocking, rectification, and anomalous conductance variations.

    Conclusions:

    • The developed kinetic model provides a framework for understanding complex single-file ion transport.
    • The model's predictions align with observed phenomena like ion blocking and rectification.
    • Further application of this theory to specific biological channels like gramicidin A is warranted.

    Related Experiment Videos