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A three-dimensional study of organelle interrelationships in regenerating rat liver. 4. Multivesicular bodies
Abstract:
Double-membraned plasma-membrane-loops are formed from the plasma membrane and released into the cytoplasm. The vesiculation of mainly their inner membrane may transform them into MVB. The so-formed internal vesicles contain cytosol. In many MVB the surface of all their internal vesicles to gether corresponds well with the surface of their bordering membrane. This correlation may be the result of a dynamic equilibrium whereby degradation (and partial recyclage) of internal vesicles is compensated for by the formation of new internal vesicles. When this equilibrium becomes disturbed, MVB may show much less internal vesicles. Such 'depleted' MVB are often producing peribiliary vesicles and may themselves transform into lacunate emptying into the bile canaliculi.
Insights
Plasma membrane-derived loops form multivesicular bodies (MVBs) containing cytosol. Disturbances in the dynamic equilibrium of internal vesicle formation and degradation can lead to depleted MVBs, impacting cellular processes.
Area of Science:
- Cell Biology
- Membrane Trafficking
- Organelle Biogenesis
Background:
- Plasma membrane-derived loops are observed in the cytoplasm.
- These structures can transform into multivesicular bodies (MVBs).
Purpose of the Study:
- To investigate the formation and transformation of plasma membrane-derived structures into MVBs.
- To explore the dynamic equilibrium governing internal vesicle content within MVBs.
- To understand the implications of disturbed equilibrium on MVB morphology and function.
Main Methods:
- Observation of plasma membrane-derived loops and MVBs.
- Analysis of internal vesicle formation and degradation dynamics.
- Characterization of 'depleted' MVB morphology and associated vesicles.
Main Results:
- Plasma membrane-derived loops can vesiculate internally to form MVBs containing cytosol.
- A dynamic equilibrium between internal vesicle degradation and formation maintains MVB surface area correlation.
- Disturbances in this equilibrium lead to depleted MVBs, which may produce peribiliary vesicles or transform into lacunate structures.
Conclusions:
- MVB formation involves the transformation of plasma membrane-derived loops.
- The internal vesicle content of MVBs is regulated by a dynamic equilibrium.
- Altered MVB equilibrium can result in distinct cellular structures and potential functional changes.