Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Serotonin release is modulated by presynaptic autoreceptors.

F Cerrito, M Raiteri

    European Journal of Pharmacology
    |August 15, 1979
    PubMed
    Summary

    This study investigated serotonin (5HT) autoreceptors in the brain. Methiothepin blocked 5HT

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Release and exchange of neurotransmitters in synaptosomes: Effects of the ionophore A23187 and of ouabain.

    Neurochemical research·2013
    Same author

    Diaminobutyric acid: A tool for discriminating between carrier-mediated and non-carrier-mediated release of GABA from synaptosomes?

    Neurochemical research·2013
    Same author

    Quantitative evaluation of two pathways for phosphatidylcholine biosynthesis in rat brain in vivo.

    Neurochemical research·2013
    Same author

    The use of a polymeric sealant for prevention of posttransplantation lymphocele.

    Transplantation proceedings·2011
    Same author

    The role of immunosuppression in malignancies among 351 pediatric renal transplant patients.

    Transplantation proceedings·2010
    Same author

    Intrastriatal kainic acid elicits functional supersensitivity of muscarinic receptors regulating dopamine release.

    Neurochemistry international·2010

    Area of Science:

    • Neuroscience
    • Neuropharmacology
    • Synaptic transmission

    Background:

    • Presynaptic autoreceptors regulate neurotransmitter release.
    • Serotonin (5HT) plays a key role in central nervous system functions.
    • Understanding 5HT autoreceptor function is crucial for neurological drug development.

    Purpose of the Study:

    • To investigate the existence and function of presynaptic autoreceptors for 5HT.
    • To differentiate between presynaptic and postsynaptic 5HT receptor actions.

    Main Methods:

    • Utilized superfused hypothalamic synaptosomes for neurotransmitter release studies.
    • Measured the release of 3H-5HT from nerve endings.
    • Employed selective 5HT receptor antagonists to block receptor activity.

    Main Results:

    • Extracellular 5HT inhibited high K+-induced 3H-5HT release from synaptosomes.
    • The 5HT receptor blocker methiothepin antagonized this inhibitory effect, indicating presynaptic autoreceptor involvement.
    • Other 5HT antagonists (cyproheptadine, methysergide, mianserin) did not inhibit 5HT release, suggesting a postsynaptic action.

    Conclusions:

    • Evidence supports the existence of presynaptic 5HT autoreceptors that inhibit 5HT release.
    • Methiothepin acts as a central 5HT receptor blocker, effective at presynaptic sites.
    • Differential antagonist activity suggests distinct roles for pre- and postsynaptic 5HT receptors.

    Related Experiment Videos