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Immunological properties of Friend virus from mouse spleen obtained by gel filtration
Abstract:
Gel filtration, with Sephadex G-200, was utilized to obtain Friend virus (FV) from infected spleen homogenate. Highly infectious and immunologically active material was eluted as a large initial protein peak, whereas hemoglobin and other noninfectious materials were found in two subsequent peaks. Fractions that made up the first peak were reactive in serological tests with antiserum towards FV. Formalinized fractions from the first peak, when combined with Freund's complete adjuvant, were able to protect mice against subsequent FV challenge. Further purification of antigen was carried out by sucrose gradient ultracentrifugation.
Insights
Researchers purified Friend virus (FV) using gel filtration, isolating an infectious and immunogenic component. This component protected mice against FV challenge, demonstrating its potential as a vaccine candidate.
Area of Science:
- Virology
- Immunology
- Biochemistry
Background:
- Friend virus (FV) is a significant pathogen in murine models.
- Effective purification methods are crucial for understanding FV's structure and function.
Purpose of the Study:
- To isolate and purify infectious and immunologically active components of Friend virus.
- To evaluate the protective efficacy of the purified FV antigen in a murine model.
Main Methods:
- Gel filtration chromatography using Sephadex G-200 to separate FV from spleen homogenate.
- Serological testing to confirm FV reactivity.
- Formalinization of purified fractions and immunization with Freund's complete adjuvant.
- Sucrose gradient ultracentrifugation for further antigen purification.
Main Results:
- A highly infectious and immunologically active FV component was successfully eluted as the first protein peak.
- This peak demonstrated reactivity in serological tests against FV antiserum.
- Immunization with formalinized fractions from the first peak conferred protection against FV challenge in mice.
Conclusions:
- Gel filtration is an effective method for purifying infectious and immunogenic Friend virus components.
- The purified FV antigen holds potential as a vaccine candidate for protection against FV infection.