Modification of a quantitative laser-spectrographic method of determination of cations contained in tissue slices

A quantitative method of determination of elements in histological slices by means of laser microanalysis has been modified. The modification consists essentially in such methodological approach that allows expressing the results by figures which are directly proportional to the relative content of the element in question. The final result, i.e. the relative quantity of the element to be determined is obtained by subtracting the photometric value yielded by the spectrographic line of the Ag standard from that yielded by the spectrographic line of the element under investigation (J = JX-JAG). To avoid negative figures the Ag standard has to be prepared in such a way, as to yield the lowest possibly photometric values still lying on the straight line of the standard curve.