Related Experiment Video
Updated: Aug 4, 2026
09:38
Identification of Nucleolar Factors During HIV-1 Replication Through Rev Immunoprecipitation and Mass Spectrometry
Published on: June 26, 2019
Structural determinant of a ribosomal protein: K locus
Science (New York, N.Y.)
|June 13, 1969
Summary
Differences in ribosomal protein 30S-8 mobility between E. coli strains are due to the protein's structural gene. This study confirms the genetic basis for electrophoretic differences in bacterial ribosomal proteins.
Area of Science:
- Molecular Biology
- Bacteriology
- Genetics
Background:
- Ribosomal proteins are essential components of bacterial ribosomes.
- Electrophoretic mobility can vary between homologous proteins from different bacterial strains.
- Escherichia coli (E. coli) is a model organism for molecular biology studies.
Purpose of the Study:
- To investigate the cause of differing electrophoretic mobilities between homologous ribosomal proteins from E. coli B and E. coli K.
- To determine if the genetic locus responsible for electrophoretic mobility is the structural gene for ribosomal protein 30S-8.
Main Methods:
- Comparative analysis of amino acid compositions of homologous ribosomal proteins.
- Analysis of tryptic peptides from ribosomal protein 30S-8.
- Analysis of cyanogen bromide peptides from ribosomal protein 30S-8.
Main Results:
- Ribosomal protein 30S-8 from E. coli B exhibited greater electrophoretic mobility than the homologous protein (30S-8K) from E. coli K.
- Amino acid composition, tryptic peptide, and cyanogen bromide peptide analyses provided unambiguous evidence.
- These analyses confirmed that the genetic locus determining electrophoretic mobility is the structural gene for protein 30S-8.
Conclusions:
- The structural gene for ribosomal protein 30S-8 dictates its electrophoretic mobility.
- Genetic variations in the structural gene are responsible for observed differences in ribosomal protein mobility between E. coli strains.
More Related Videos
Related Concept Videos
01:27Ribosomes
Ribosomes translate genetic information encoded by messenger RNA (mRNA) into proteins. Both prokaryotic and eukaryotic cells have ribosomes. Cells that synthesize large quantities of protein—such as secretory cells in the human pancreas—can contain millions of ribosomes.Ribosome Structure and AssemblyRibosomes are composed of ribosomal RNA (rRNA) and proteins. In eukaryotes, rRNA is transcribed from genes in the nucleolus—a part of the nucleus that specializes in ribosome production. Within the...
01:23Types of RNA
Overview
Three main types of RNA are involved in protein synthesis: messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). These RNAs perform diverse functions and can be broadly classified as protein-coding or non-coding RNA. Non-coding RNAs play important roles in the regulation of gene expression in response to developmental and environmental changes. Non-coding RNAs in prokaryotes can be manipulated to develop more effective antibacterial drugs for human or animal use.
RNA...
Three main types of RNA are involved in protein synthesis: messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). These RNAs perform diverse functions and can be broadly classified as protein-coding or non-coding RNA. Non-coding RNAs play important roles in the regulation of gene expression in response to developmental and environmental changes. Non-coding RNAs in prokaryotes can be manipulated to develop more effective antibacterial drugs for human or animal use.
RNA...
01:56Riboswitches
Riboswitches are non-coding mRNA domains that regulate the transcription and translation of downstream genes without the help of proteins. Riboswitches bind directly to a metabolite and can form unique stem-loop or hairpin structures in response to the amount of the metabolite present. They have two distinct regions – a metabolite-binding aptamer and an expression platform.
The aptamer has high specificity for a particular metabolite which allows riboswitches to specifically regulate...
The aptamer has high specificity for a particular metabolite which allows riboswitches to specifically regulate...
02:24Ribosome Profiling
Ribosome profiling or ribo-sequencing is a deep sequencing technique that produces a snapshot of active translation in a cell. It selectively sequences the mRNAs protected by ribosomes to get an insight into a cell’s translation landscape at any given point in time.
Applications of ribosome profiling
Ribosome profiling has many applications, including in vivo monitoring of translation inside a particular organ or tissue type and quantifying new protein synthesis levels.
The technique helps...
Applications of ribosome profiling
Ribosome profiling has many applications, including in vivo monitoring of translation inside a particular organ or tissue type and quantifying new protein synthesis levels.
The technique helps...
01:23Transcriptional Regulation: Riboswitches
Riboswitches are RNA elements that regulate gene expression by altering their secondary structures in response to specific effector molecules. These elements, located in the leader regions of certain mRNAs, act as transcriptional regulators by toggling between alternative conformations to control downstream gene expression. Riboswitch-mediated regulation is a precise mechanism for modulating biosynthetic pathways, as exemplified by the riboflavin biosynthesis pathway in Bacillus...
01:29Translational Regulation
Translational regulation in prokaryotes ensures efficient protein synthesis by controlling ribosome access to mRNA. This regulation is mediated by secondary RNA structures, including translational riboswitches, RNA thermometers, and small RNAs (sRNAs), which respond to intracellular and environmental signals to modulate gene expression.Translational RiboswitchesRiboswitches in the leader region of mRNAs can regulate translation by altering the accessibility of the Shine-Dalgarno (SD) sequence,...