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Interferon production: variation in yields from human cell lines.

A Meager, H E Graves, J Shuttleworth

    Infection and Immunity
    |August 1, 1979
    PubMed
    Summary
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    Human cell lines show varied interferon production capacity. Priming and superinduction techniques can enhance yields, but results differ across cell types, impacting interferon research and therapeutic development.

    Area of Science:

    • Immunology
    • Cell Biology
    • Virology

    Background:

    • Interferon (IFN) production is crucial for antiviral defense.
    • Understanding cell line variability in IFN production is key for research applications.
    • Polyriboinosinic:polycytidylic acid (PI:RC) and Newcastle disease virus (NDV) are common IFN inducers.

    Purpose of the Study:

    • To assess the interferon production capacity of various human cell lines.
    • To investigate the effects of priming and superinduction on interferon yields.
    • To compare interferon induction by PI:RC and NDV across different cell types.

    Main Methods:

    • Surveyed human diploid, aneusomic, transformed, and tumor fibroblast cell lines.
    • Induced interferon production using polyriboinosinic:polycytidylic acid (PI:RC).

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  • Investigated the impact of priming with human interferon and superinduction techniques.

    • Main Results:

    • Significant variation in interferon production was observed among cell lines, with no clear correlation to karyotype or phenotype.
    • Priming cells with human interferon generally increased PI:RC-induced interferon yields.
    • Superinduction also enhanced yields, and combined priming and superinduction produced high yields in some lines, but not all.

    Conclusions:

    • Human cell lines exhibit diverse responses to interferon induction.
    • Priming and superinduction are effective strategies to boost interferon production, though efficacy varies.
    • Interferon production capacity after PI:RC induction does not reliably predict response to NDV induction.