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Mixed lymphocyte reactions and tissue transplantation tolerance
Science (New York, N.Y.)
|February 27, 1970
Summary
Neonatal inoculation with bone marrow cells induces tolerance to Lewis histocompatibility antigens in BN rats, demonstrated by prolonged skin graft survival and reduced mixed lymphocyte reaction. Lymph node and thymocyte inoculations did not confer tolerance.
Area of Science:
- Immunology
- Transplantation Biology
- Histocompatibility
Background:
- Histocompatibility antigens play a crucial role in transplantation success.
- Understanding antigen expression on different cell types is key to inducing immune tolerance.
Purpose of the Study:
- To investigate the induction of tolerance to Lewis histocompatibility antigens in BN rats.
- To determine the role of different cell types (bone marrow, lymph node, thymocytes) in inducing tolerance.
Main Methods:
- BN rats were inoculated neonatally with BNI Lewis F(1) hybrid bone marrow cells, lymph node cells, or thymocytes.
- Prolonged survival of Lewis skin grafts was assessed.
- Mixed lymphocyte interaction assays were performed to measure immune reactivity.
Main Results:
- Bone marrow cell inoculation led to prolonged Lewis skin graft survival and decreased mixed lymphocyte reactivity, indicating tolerance.
- Lymph node cell inoculation diminished mixed lymphocyte reactivity but did not induce skin graft tolerance.
- Thymocyte inoculation failed to induce unresponsiveness in vitro or skin graft tolerance.
Conclusions:
- Bone marrow cells effectively induce tolerance to Lewis histocompatibility antigens in rats.
- Histocompatibility antigens are differentially expressed, with bone marrow cells carrying antigens absent or poorly expressed on lymph node cells and thymocytes.