Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Prostaglandin F2alpha and human prostatic affinity for testosterone.

W E Farnsworth, J W Wilks

    Prostaglandins
    |January 1, 1975
    PubMed
    Summary

    Prostaglandin F2alpha enhances testosterone binding in the prostate, contrary to expectations. Its interaction with lactogen is complex, with specific concentrations showing effects and stromal tissue appearing to be the primary site of prostaglandin action.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Estrogen in the etiopathogenesis of BPH.

    The Prostate·1999
    Same author

    Synthesis of a series of stromelysin-selective thiadiazole urea matrix metalloproteinase inhibitors.

    Journal of medicinal chemistry·1999
    Same author

    Prostate stroma: physiology.

    The Prostate·1999
    Same author

    Structural characterizations of nonpeptidic thiadiazole inhibitors of matrix metalloproteinases reveal the basis for stromelysin selectivity.

    Protein science : a publication of the Protein Society·1998
    Same author

    Synthetic matrix metalloproteinase inhibitors and tissue inhibitor of metalloproteinase (TIMP)-2, but not TIMP-1, inhibit shedding of tumor necrosis factor-alpha receptors in a human colon adenocarcinoma (Colo 205) cell line.

    Cancer research·1998
    Same author

    Figuring out why we breathe.

    Medical hypotheses·1997

    Area of Science:

    • Endocrinology
    • Reproductive Biology
    • Prostate Physiology

    Background:

    • Lactogens, such as luteotropic hormone (LTH) and human placental lactogen (HPL), are known to promote testosterone binding in the prostate.
    • Prostaglandin F2alpha (PGF2alpha) has a known antagonistic effect on the luteotrophic action of hormones on the corpus luteum.

    Purpose of the Study:

    • To investigate whether prostaglandin F2alpha opposes the effect of lactogens on prostate testosterone binding.
    • To characterize the specific effects of prostaglandin F2alpha on prostate steroid binding.

    Main Methods:

    • Administration of varying concentrations of prostaglandin F2alpha to prostate tissue.
    • Assessment of testosterone binding in response to lactogen and prostaglandin F2alpha.
    • Analysis of prostaglandin F2alpha concentrations in prostate tissue extracts and correlation with beta-glucuronidase activity.
    Keywords:
    AndrogensBiologyEndocrine SystemExaminations And DiagnosesGenitaliaGenitalia, MaleHistologyHormonesLaboratory Examinations And DiagnosesLaboratory ProceduresPhysiologyProstaglandins--analysisProstaglandins--side effectsProstateTestosteroneUrogenital System

    Related Experiment Videos

    Main Results:

    • Prostaglandin F2alpha was found to increase steroid binding, rather than oppose lactogen's effect.
    • The interaction between prostaglandin F2alpha and lactogen was neither antagonistic nor additive.
    • Optimal enhancement of binding occurred at 4 ng/ml and 40 ng/ml of F2alpha; higher concentrations (400 ng/ml) were ineffective.
    • Prostate glands with stromal hyperplasia/inflammation showed different responsiveness compared to those with epithelial hyperplasia.
    • Tissue assays indicated approximately 340 ng of F2alpha per gram of fresh weight, with concentration inversely related to beta-glucuronidase activity.

    Conclusions:

    • Prostaglandin F2alpha enhances prostate steroid binding, with a complex interaction with lactogens.
    • The findings suggest a stromal localization for prostaglandin concentration within the prostate, potentially linked to tissue hyperplasia and inflammation.