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Related Experiment Videos

Complement components in normal serum and plasma quantitated by electroimmunoassay.

A G Sjöholm

    Scandinavian Journal of Immunology
    |January 1, 1975
    PubMed
    Summary

    This study quantifies complement components in normal serum and plasma using electroimmunoassay, providing normal ranges and insights into protein interdependencies. Results show good reproducibility and stability under various storage conditions.

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    Area of Science:

    • Immunology
    • Clinical Chemistry

    Background:

    • The human complement system is crucial for innate immunity.
    • Accurate quantification of complement components is essential for diagnosing and monitoring various diseases.
    • Electroimmunoassay offers a sensitive method for measuring complement protein concentrations.

    Purpose of the Study:

    • To determine the normal concentration ranges for key complement components in healthy adults.
    • To investigate the interrelationships between different complement proteins.
    • To assess the reproducibility and stability of complement component measurements.

    Main Methods:

    • Electroimmunoassay was employed to quantify C1q, C1s, C3, C4, C5, C3 proactivator, and C1 inactivator.
    • Serum and EDTA plasma samples from 100 normal adults were analyzed.

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  • Reproducibility, inter-individual variation, and effects of sample handling (freeze-thaw, storage) were evaluated.

    • Main Results:

    • Normal ranges for each quantified complement component were established.
    • C1q and C1s levels showed a strong correlation, as did C3, C5, and C3 proactivator.
    • Electroimmunoassay demonstrated good reproducibility (4.9%–7.9%) and agreement with single radial immunodiffusion for C3 and C4.

    Conclusions:

    • Electroimmunoassay is a reliable method for quantifying complement components in serum and plasma.
    • Understanding normal ranges and protein interdependencies aids in interpreting complement-related findings.
    • Complement component levels are relatively stable under common laboratory handling and storage conditions.