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Related Experiment Videos

Alterations in human intestinal microflora during experimental diarrhoea.

S L Gorbach, G Neale, R Levitan

    Gut
    |January 1, 1970
    PubMed
    Summary

    Fluid administration and diarrhea alter gut bacteria, allowing colonic bacteria like Enterobacter to overgrow in the small intestine. This overgrowth can be reduced by addressing underlying issues like pancreatic insufficiency.

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    Area of Science:

    • Gastroenterology
    • Microbiology
    • Human Physiology

    Background:

    • The small and large bowel harbor distinct bacterial communities.
    • Disruptions to normal bowel function can alter this delicate balance.

    Purpose of the Study:

    • To investigate the impact of fluid administration and experimental diarrhea on small and large bowel bacteriology.
    • To understand the role of Enterobacter species in altered gut flora.

    Main Methods:

    • Administration of large fluid volumes via intestinal tube.
    • Induction of experimental diarrhea (fluid purgation, pancreatic enzyme withdrawal, lactose feeding).
    • Analysis of fecal and small bowel microflora composition.

    Main Results:

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    • Fluid administration led to the presence of colonic bacteria in the small bowel of normal subjects.
    • Diarrhea induced by various methods altered fecal microflora, often increasing Enterobacter species.
    • In pancreatic insufficiency, Enterobacter fecal concentration correlated with bowel movement frequency, decreasing with enzyme treatment.
    • Enterobacter were found in the small intestine and suppressed in the large bowel during normal function, with diarrhea removing this suppression.

    Conclusions:

    • Experimental diarrhea and fluid purging significantly alter gut bacteriology, particularly increasing Enterobacter species.
    • These bacteriological shifts mimic changes observed in naturally occurring diarrheal diseases.
    • Maintaining normal bowel function is crucial for suppressing potentially pathogenic bacteria like Enterobacter in the small intestine.