Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Heat-sensitive early function in induced lambda Nsus lysogens.

R A Cross, M Lieb

    Journal of Virology
    |July 1, 1970
    PubMed
    Summary
    This summary is machine-generated.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    [Alzheimer dementia: course and burden on caregivers : Data over 18 months from German participants of the GERAS study].

    Der Nervenarzt·2017
    Same author

    Review: Mechanochemistry of the kinesin-1 ATPase.

    Biopolymers·2016
    Same author

    Recent Developments in Pharmacotherapy of Alcoholism.

    Pharmacopsychiatry·2015
    Same author

    [Electroconvulsive therapy - indications and practical use for pharmacotherapy resistant depressive disorders].

    Fortschritte der Neurologie-Psychiatrie·2012
    Same author

    [Schizophrenia: more than just a "disturbance of the integrity of the self"].

    Der Nervenarzt·2012
    Same author

    [Psychotic and non-psychotic denial of pregnancy].

    Fortschritte der Neurologie-Psychiatrie·2011
    Same journal

    Dysregulation of miRNAs has broad impacts on virus infection in <i>Drosophila</i>.

    Journal of virology·2026
    Same journal

    Identification of GRP78 as a novel host factor that facilitates zoonotic porcine deltacoronavirus internalization and replication via clathrin-mediated endocytosis.

    Journal of virology·2026
    Same journal

    MGF110-2L deletion prevents IFN-I and inflammatory response, resulting in partial attenuation and protection against virulent ASFV.

    Journal of virology·2026
    Same journal

    Periodic genome sequences facilitate packaging in a single-stranded DNA virus.

    Journal of virology·2026
    Same journal

    A novel monoclonal antibody targeting the hemagglutinin-neuraminidase of peste des petits ruminants virus maintains neutralizing activity by blocking viral adsorption and receptor interaction.

    Journal of virology·2026
    Same journal

    ATG9A is an essential host factor for parechovirus RNA replication.

    Journal of virology·2026
    See all related articles

    Mutations in bacteriophage lambda gene N prevent host killing. Derepression of Nsus prophages in nonpermissive bacteria causes cell death, but rescue is possible via superinfection, independent of lysogenization.

    Area of Science:

    • Bacteriophage biology
    • Molecular genetics
    • Microbial genetics

    Background:

    • Bacteriophage lambda gene N is crucial for host bacterium lysis.
    • Derepression of Nsus prophages in nonpermissive bacteria leads to lysogen cell death.
    • Conditional mutations affect phage behavior under specific environmental conditions.

    Purpose of the Study:

    • Investigate the rescue mechanism of heat-inducible lambda lysogens.
    • Determine the role of superinfection and lysogenization in cell viability.
    • Analyze the impact of temperature shifts on deoxyribonucleic acid (DNA) synthesis and rescue potential.

    Main Methods:

    • Utilizing nonpermissive (pm(-)) bacteria harboring lambdaCItsA-Nsus lysogens.
    • Inducing prophage derepression by temperature shift to 45 C.

    Related Experiment Videos

  • Assessing cell viability and colony formation at permissive (33 C) and nonpermissive (45 C) temperatures.
  • Monitoring deoxyribonucleic acid (DNA) synthesis rates under different temperature conditions.
  • Investigating the effect of lambdaCI(+)-Nsus superinfection on cell rescue.

    • Main Results:

    • Cells remain viable at 45 C after prophage derepression but cannot form colonies at 33 C.
    • Superinfection with lambdaCI(+)-Nsus phage at 45 C rescues heat-inducible lysogens, enabling colony formation at 33 C.
    • Lysogenization by the superinfecting phage is not essential for rescue.
    • A temperature downshift from 45 C to 33 C in pm(-) (lambdaCItsA-Nsus) lysogens triggers increased DNA synthesis, correlating with loss of rescue potential.
    • Sustained incubation at 45 C prevents this increase in DNA synthesis and maintains rescue potential.

    Conclusions:

    • Bacteriophage lambda Nsus prophage derepression in nonpermissive conditions is lethal.
    • Rescue of heat-inducible lysogens is possible through superinfection, independent of subsequent lysogenization.
    • Temperature-sensitive regulation of DNA synthesis is linked to the loss of rescue potential in lambda lysogens.