Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Lipid composition of Staphylococcus aureus and its derived L-forms.

M Hayami, A Okabe, R Kariyama

    Microbiology and Immunology
    |January 1, 1979
    PubMed
    Summary

    Staphylococcus aureus L-forms, unlike parent bacteria, show significantly higher cardiolipin levels and can synthesize cholesterol. These lipid changes suggest adaptation following cell wall loss.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Molecular Cytogenetic Characterization of a Karyotype of a Female Patient with Secondary Amenorrhea with a Cell Line Showing 46,X,+mar.

    Journal of the Association of Genetic Technologists·2019
    Same author

    Problems associated with prophylactic use of erythromycin in 1566 staff to prevent hospital infection during the outbreak of pertussis.

    Journal of clinical pharmacy and therapeutics·2010
    Same author

    Breed differentiation among Japanese native chickens by specific skull features determined by direct measurements and computer vision techniques.

    British poultry science·2008
    Same author

    Glycine receptors mediate excitation of subplate neurons in neonatal rat cerebral cortex.

    Journal of neurophysiology·2008
    Same author

    Single extreme low dose/low dose rate irradiation causes alteration in lifespan and genome instability in primary human cells.

    British journal of cancer·2007
    Same author

    Chromosomal aberrations in normal human cells induced by the auger effect via Ca atoms.

    International journal of radiation biology·2005

    Area of Science:

    • Microbiology
    • Biochemistry
    • Cell Biology

    Background:

    • Staphylococcus aureus can exist as L-forms, which lack a cell wall.
    • Understanding lipid composition differences between bacterial strains and their L-forms is crucial for comprehending cellular adaptation.

    Purpose of the Study:

    • To analyze and compare the lipid compositions of Staphylococcus aureus parent strains and their derived L-forms.
    • To investigate the synthesis of cholesterol and its esters by Staphylococcus aureus L-forms.

    Main Methods:

    • Culturing of Staphylococcus aureus (Newman and Tazaki strains) and their L-forms in serum-containing and serum-free broths.
    • Analysis of lipid composition, focusing on phospholipids (cardiolipin) and cholesterol content.

    Main Results:

    Related Experiment Videos

    • L-forms exhibited significantly higher cardiolipin content (>50%) compared to parent bacteria (<25%).
    • Both parent strains and L-forms incorporated serum cholesterol, but L-forms also synthesized cholesterol and its esters independently, especially in serum-free conditions.

    Conclusions:

    • Staphylococcus aureus L-forms undergo substantial modifications in membrane lipid composition, notably increased cardiolipin and cholesterol synthesis.
    • These lipid alterations likely represent an adaptive response to the absence of a cell wall.