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Related Experiment Videos

Filterability of streptococcal L-forms.

P B Wyrick, H Gooer

    Journal of Bacteriology
    |January 1, 1971
    PubMed
    Summary
    This summary is machine-generated.

    Streptococcus faecium L-forms and protoplasts can pass through 0.45-micrometer filters, indicating their small size. Parent bacteria are retained by larger filters, aiding in L-form characterization.

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    Area of Science:

    • Microbiology
    • Bacteriology

    Background:

    • Streptococcus faecium can exist in a stable L-form state, lacking a cell wall.
    • L-forms and protoplasts are significantly smaller than their parent bacterial cells.

    Purpose of the Study:

    • To determine the filterability of Streptococcus faecium L-forms and protoplasts.
    • To characterize the size of L-forms and protoplasts through filtration experiments.

    Main Methods:

    • Filtration of broth-grown L-forms and protoplasts using various pore sizes (0.45 µm, 0.22 µm, 1.2 µm).
    • Filtration of parent Streptococcus faecium cells as a control.
    • Incubation of filtrates to assess the viability of recovered L-forms and bacteria.

    Main Results:

    • Viable L-forms were recovered from 0.45-µm filtrates, but not 0.22-µm filtrates.

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  • Protoplasts passed through filters of 0.45 µm or greater.
  • Parent Streptococcus faecium cells were only recovered from 1.2-µm filtrates.

    • Conclusions:

    • Streptococcus faecium L-forms and protoplasts are capable of passing through 0.45-µm filters.
    • Filtration is a viable method for separating L-forms from parent bacterial cells based on size.