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Lambda mutants which persist as plasmids.

M Lieb

    Journal of Virology
    |August 1, 1970
    PubMed
    Summary
    This summary is machine-generated.

    Mutated lambda phages persist as plasmids in host bacteria instead of killing them. These lambda plasmids replicate independently and can be carried by bacteria without immunity, showing complementation with other phages.

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    Area of Science:

    • Bacteriology
    • Molecular Biology
    • Virology

    Background:

    • Lambda phages are viruses that infect bacteria.
    • Gene N mutations in lambda phages prevent host cell lysis.
    • Lambda phage genomes can exist as plasmids within host bacteria.

    Purpose of the Study:

    • To investigate the persistence and replication of lambda phage genomes in host bacteria.
    • To characterize the behavior of mutated lambda phages that form plasmids.
    • To understand the relationship between lambda plasmids and host immunity.

    Main Methods:

    • Infection of sensitive host bacteria with lambda phages mutated in gene N.
    • Analysis of plasmid formation and replication using genetic markers (cI, sus, ts, c).
    • Co-infection experiments with multiple phage particles and co-existence with F plasmids.

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  • Assessment of bacterial immunity and complementation between plasmids and superinfecting phages.

    • Main Results:

    • Lambda phages mutated in gene N do not kill host bacteria but form plasmids.
    • Plasmids are formed by various lambda genome mutants, including cI(+), sus, ts, and c.
    • Infected bacteria become carriers, with 10-20 lambda plasmids per host genome.
    • Lambda and F plasmids replicate independently in co-infected bacteria.
    • Lambda plasmid carriers are not immune and exhibit complementation with superinfecting lambda mutants.

    Conclusions:

    • Lambda phage gene N mutations enable plasmid formation and bacterial carrier states.
    • Lambda plasmids replicate autonomously and independently of host F plasmids.
    • The absence of immunity in carriers and complementation suggest complex interactions within the host.