Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Conformational changes in ribosomes during protein synthesis.

M H Schreier, H Noll

    Proceedings of the National Academy of Sciences of the United States of America
    |April 1, 1971
    PubMed
    Summary

    Ribosomes contract during aminoacyl-tRNA binding and expand during translocation, a cycle essential for protein synthesis. This process, involving transfer factors T and G, is crucial even for initiating peptide bond formation.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Aromatic quinolinecarboxamides as selective, orally active antibody production inhibitors for prevention of acute xenograft rejection.

    Journal of medicinal chemistry·2001
    Same author

    Inhibition of cyclosporin-resistant B-cell antigen responses by pyrazoles: a tool for the identification of novel molecular mechanisms of B-cell activation.

    Transplantation proceedings·2001
    Same author

    Prevention of acute allograft rejection in nonhuman primate lung transplant recipients: induction with chimeric anti-interleukin-2 receptor monoclonal antibody improves the tolerability and potentiates the immunosuppressive activity of a regimen using low doses of both microemulsion cyclosporine and 40-O-(2-hydroxyethyl)-rapamycin.

    Transplantation·2000
    Same author

    Isoxazolylthioamides as potential immunosuppressants a combinatorial chemistry approach.

    Bioorganic & medicinal chemistry letters·1999
    Same author

    Relative affinity of CHI 621 for T-cell blasts of humans, rhesus, cynomolgus monkeys and dogs.

    Transplantation proceedings·1998
    Same author

    Pyrazole bioisosteres of leflunomide as B-cell immunosuppressants for xenotransplantation and chronic rejection: scope and limitations.

    Journal of medicinal chemistry·1998

    Area of Science:

    • Molecular Biology
    • Protein Synthesis
    • Ribosome Dynamics

    Background:

    • The Escherichia coli ribosome undergoes conformational changes during protein synthesis.
    • Transfer factors T and G play critical roles in ribosomal function.

    Purpose of the Study:

    • To elucidate the dynamic conformational changes of the ribosome during amino acid addition and translocation.
    • To investigate the role of transfer factors T and G in ribosome contraction and expansion.
    • To model early stages of protein synthesis initiation in the absence of initiation factors.

    Main Methods:

    • Utilized a purified system with Escherichia coli ribosomal subunits, poly(U), transfer factors T and G, and aminoacyl-tRNA.
    • Observed ribosomal complex conformational changes (contraction and expansion) during the elongation cycle.
    • Investigated the requirement of translocation by factor G for the first peptide bond formation.

    Main Results:

    • Aminoacyl-tRNA binding (factor T) stabilizes a compact 70S ribosomal conformation.
    • Translocation (factor G) expands the ribosome to a less stable 60S form.
    • Translocation by factor G is necessary before the first peptide bond can form.

    Conclusions:

    • Ribosome conformation dynamically cycles between compact (70S) and expanded (60S) states during elongation.
    • Factor G-mediated translocation is a prerequisite for initiating peptide bond formation, even in simplified systems.
    • Findings support a model of initiation involving deacylated tRNA and factor G-mediated translocation.

    Related Experiment Videos