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Related Experiment Videos

Metachromatic agar-diffusion methods for detecting staphylococcal nuclease activity.

R V Lachica, C Genigeorgis, P D Hoeprich

    Applied Microbiology
    |April 1, 1971
    PubMed
    Summary
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    New methods detect Staphylococcus aureus nuclease using Toluidine Blue O. These agar-diffusion techniques identify the bacteria at low concentrations in cultures, aiding in rapid diagnostics.

    Area of Science:

    • Microbiology
    • Biochemistry

    Background:

    • Staphylococcus aureus is a significant pathogen.
    • Accurate detection of bacterial nucleases is crucial for diagnostics.

    Purpose of the Study:

    • To develop convenient agar-diffusion methods for detecting Staphylococcus aureus nuclease.
    • To investigate the interactions of agar, deoxyribonucleic acid, and Toluidine Blue O.

    Main Methods:

    • Utilized the metachromatic property of Toluidine Blue O.
    • Developed three distinct agar-diffusion assays.
    • Tested detection limits in agar and broth cultures.

    Main Results:

    • Successfully detected Staphylococcus aureus nuclease.
    • Achieved detection as low as 0.005 mug/ml.

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  • Demonstrated the utility of Toluidine Blue O in these assays.

    • Conclusions:

    • The developed methods offer a convenient and sensitive approach for identifying Staphylococcus aureus nuclease.
    • Toluidine Blue O's metachromatic properties are effective for this detection.
    • These methods can aid in the rapid identification of Staphylococcus aureus.