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Herpes simplex virus type 1 replication in a gingival cell line.

R D Fletcher, J Guggenheimer, D Sumney

    Journal of Dental Research
    |May 1, 1975
    PubMed
    Summary
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    Gingival cells are superior to HeLa cells for studying herpes simplex virus type 1 (HSV-1) effects. These cells offer a clear, cost-effective model for observing viral cytopathic effects and oral tissue responses to HSV-1.

    Area of Science:

    • Virology
    • Cell Biology
    • Oral Pathology

    Background:

    • Herpes simplex virus type 1 (HSV-1) is a common human pathogen.
    • Understanding HSV-1 cytopathic effects is crucial for diagnosing and managing infections.
    • Current models for studying HSV-1 may have limitations in accurately reflecting oral tissue responses.

    Purpose of the Study:

    • To evaluate the utility of gingival cells as a model for HSV-1 propagation and cytopathic effect analysis.
    • To compare the effectiveness of gingival cells versus HeLa cells in demonstrating HSV-1-induced cytopathology.
    • To establish a cost-effective and definitive laboratory model for studying oral tissue reactions to HSV-1.

    Main Methods:

    • Propagation of HSV-1 in primary human gingival cell cultures.

    Related Experiment Videos

  • Inoculation of gingival cells with HSV-1 from standard laboratory cultures and clinical isolates.
  • Microscopic examination to characterize viral-induced cytopathic effects (CPE).
  • Comparison of CPE in gingival cells with those observed in HeLa cells.

    • Main Results:

    • Gingival cells demonstrated clear and characteristic HSV-1-induced cytopathic effects.
    • These effects were more definitive in gingival cells compared to HeLa cells.
    • Gingival cells proved to be easily and economically maintained in laboratory settings.
    • The model provides a suitable platform for observing oral tissue reactions to HSV-1.

    Conclusions:

    • Gingival cells serve as an advantageous and definitive cell culture model for studying HSV-1.
    • This model offers a cost-effective and reliable method for characterizing viral cytopathology.
    • Gingival cells provide a valuable tool for investigating oral tissue responses to HSV-1 infection.