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An intestinal receptor for heme.

R Gräsbeck, I Kouvonen, M Lundberg

    Scandinavian Journal of Haematology
    |July 1, 1979
    PubMed
    Summary
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    Researchers identified a high-affinity heme binder in the upper small intestine of pigs. This brush border protein specifically binds heme, not iron, and is also present in humans.

    Area of Science:

    • Biochemistry
    • Gastroenterology
    • Molecular Biology

    Background:

    • Heme absorption is crucial for iron homeostasis.
    • The specific mechanisms and binding proteins involved in intestinal heme uptake are not fully elucidated.

    Purpose of the Study:

    • To investigate the presence and characteristics of a heme-binding factor in the porcine upper small intestine.
    • To determine the localization and binding properties of this factor.

    Main Methods:

    • In vitro uptake studies using radioactive heme with porcine intestinal tissue.
    • Solubilization of the binding factor using Triton X-100.
    • Competitive binding assays with non-radioactive heme and ferrous sulfate (FeSO4).
    • Control experiments to rule out known heme binders and aggregated heme.

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    Main Results:

    • Porcine upper small intestine demonstrated in vitro uptake of radioactive heme.
    • The heme binder was localized to the brush borders.
    • The binder was solubilized and exhibited high-affinity heme binding.
    • Binding was specifically inhibited by non-radioactive heme, but not by FeSO4.
    • Control experiments excluded known heme binders and aggregated heme.

    Conclusions:

    • A novel high-affinity heme-binding factor exists in the brush border of the porcine upper small intestine.
    • This factor plays a role in heme uptake and is distinct from simple iron binding.
    • The presence of a similar binding factor in the human duodenum suggests conserved mechanisms for heme absorption.