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Multi-biochemical test system for distinguishing enteric and other gram-negative bacilli.

H R Elston, J A Baudo, J P Stanek

    Applied Microbiology
    |September 1, 1971
    PubMed
    Summary
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    The Enterotube system offers a convenient method for identifying gram-negative bacilli but shows discrepancies in several biochemical tests. It requires further validation for accurate bacterial identification, especially for Shigella and Salmonella species.

    Area of Science:

    • Clinical microbiology
    • Bacteriology
    • Diagnostic testing

    Background:

    • Accurate identification of enteric and gram-negative bacilli is crucial for patient diagnosis and treatment.
    • Conventional biochemical tests are time-consuming and labor-intensive.
    • Novel multi-biochemical test systems aim to simplify and expedite bacterial identification.

    Purpose of the Study:

    • To evaluate the performance of the Enterotube multi-biochemical test system.
    • To compare the Enterotube's accuracy against conventional methods for identifying enteric and gram-negative bacilli.
    • To assess the Enterotube's utility in clinical microbiology settings.

    Main Methods:

    • A total of 242 bacterial strains from various sources were tested.
    • The Enterotube system was used in parallel with conventional biochemical tests.

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  • Results from individual tests and overall identification were compared between the two systems.

    • Main Results:

    • High agreement was observed for indole and dextrose tests.
    • Significant discrepancies were noted in hydrogen sulfide, phenylalanine, urease, dulcitol, lactose, lysine decarboxylase, and citrate tests.
    • The Enterotube system incorrectly identified 15 strains and failed to differentiate 4 closely related strains.
    • Lysine decarboxylase test interpretation was problematic.
    • Salmonella could be identified to group, but Shigella was often misidentified as Escherichia.

    Conclusions:

    • The Enterotube system is simple and convenient for simultaneous inoculation.
    • However, discrepancies in several biochemical tests and identification errors limit its reliability.
    • The Enterotube requires further validation and may not be a complete replacement for conventional methods, particularly for specific genera like Shigella.