Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Superinfection exclusion by P22 prophage and the replication complex.

O F Darlington, M Levine

    Journal of Virology
    |September 1, 1971
    PubMed
    Summary
    This summary is machine-generated.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Different core promoters possess distinct regulatory activities in the Drosophila embryo.

    Genes & development·1998
    Same author

    Rush to judgment? Child protective services and allegations of sexual abuse.

    The American journal of orthopsychiatry·1998
    Same author

    In vivo cimetidine inhibits hepatic CYP2C6 and CYP2C11 but not CYP1A1 in adult male rats.

    The Journal of pharmacology and experimental therapeutics·1998
    Same author

    Pulmonary toxicity of high-dose chemotherapy for breast cancer: a non-invasive approach to diagnosis and treatment.

    Bone marrow transplantation·1998
    Same author

    Ascorbate recycling in human neutrophils: induction by bacteria.

    Proceedings of the National Academy of Sciences of the United States of America·1998
    Same author

    The occurrence of subsequent malignancy in patients presenting with deep vein thrombosis: results from a historical cohort study.

    Thrombosis and haemostasis·1998

    Wild-type P22 prophage lysogens prevent superinfecting phage deoxyribonucleic acid (DNA) injection. In contrast, mutant lysogens allow superinfecting phage DNA to associate with the replication complex, revealing a key difference in phage exclusion mechanisms.

    Area of Science:

    • Bacteriology
    • Molecular Biology
    • Virology

    Background:

    • Bacteriophage P22 is a model system for studying lysogenic conversion and phage-host interactions.
    • Lysogenic conversion involves the modification of a host bacterium's properties by prophage genes.
    • Phage exclusion is a critical mechanism for maintaining lysogeny and preventing superinfection.

    Purpose of the Study:

    • To investigate the role of the sie gene in P22-mediated phage exclusion.
    • To determine the fate of superinfecting phage deoxyribonucleic acid (DNA) in wild-type versus mutant lysogens.
    • To elucidate the molecular mechanisms underlying P22-induced exclusion of superinfecting phage DNA.

    Main Methods:

    • Salmonella typhimurium strains lysogenized with wild-type (sie(+)) or mutant (sie(-)) P22 prophage were constructed.

    Related Experiment Videos

  • Superinfection experiments were performed using virulent P22 phage.
  • Association of superinfecting phage DNA with the bacterial replication complex was analyzed using biochemical methods.

    • Main Results:

    • Wild-type P22 lysogens effectively excluded deoxyribonucleic acid (DNA) from superinfecting phage.
    • In contrast, sie(-) P22 lysogens failed to exclude superinfecting phage DNA.
    • Superinfecting phage DNA associated with the replication complex in sie(-) lysogens but not in sie(+) lysogens.

    Conclusions:

    • The sie gene product of P22 prophage is essential for mediating exclusion of superinfecting phage DNA.
    • Sie-mediated exclusion likely involves preventing the association of superinfecting DNA with the host replication machinery.
    • This study clarifies a key aspect of P22 phage-host interactions and the maintenance of lysogeny.