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Related Experiment Videos

Control of lambda repressor synthesis.

L Reichardt, A D Kaiser

    Proceedings of the National Academy of Sciences of the United States of America
    |September 1, 1971
    PubMed
    Summary
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    This study measured lambda repressor levels, revealing two promoters (prm and pre) control its synthesis. Promoter pre is crucial for lysogeny establishment, while prm maintains repressor in established lysogens.

    Area of Science:

    • Molecular Biology
    • Genetics
    • Virology

    Background:

    • Bacteriophage lambda repressor (cI) is essential for maintaining lysogeny.
    • Understanding repressor synthesis regulation is key to phage-host interactions.

    Purpose of the Study:

    • To directly measure intracellular lambda repressor levels.
    • To elucidate the regulatory mechanisms controlling cI gene transcription.

    Main Methods:

    • Direct measurement using DNA-filter assay and radioimmune assay.
    • Genetic analysis of phage mutants affecting repressor synthesis.
    • Deletion mapping to localize regulatory elements.

    Main Results:

    • Identified two transcription initiation sites for cI: prm and pre.

    Related Experiment Videos

  • Demonstrated that promoter pre activation requires cII and cIII proteins at site cY for lysogeny.
  • Showed that Cro protein inhibits repressor synthesis during the infective cycle.
  • Established that promoter prm, located near the right operator (Or), is activated by repressor binding to Or and maintains repressor in lysogens.

    • Conclusions:

    • Lambda repressor synthesis is tightly regulated by distinct promoters and regulatory proteins.
    • Efficient lysogeny depends on the coordinated action of cII, cIII, and promoter pre.
    • Promoter prm ensures stable lysogeny through repressor auto-activation at the right operator.