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A simple staining method for vaginal smears using red ink.

R H Davis, D L Kramer, J W Sackman

    Laboratory Animal Science
    |June 1, 1975
    PubMed
    Summary
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    This study accurately classified menstrual cycle phases in rhesus monkeys using a simple, quick, and inexpensive red ink staining technique for vaginal smears. The method reliably identified cellular changes throughout the 37 menstrual cycles observed.

    Area of Science:

    • Primate reproductive endocrinology
    • Comparative reproductive biology
    • Non-human primate models in research

    Background:

    • Accurate classification of menstrual cycle phases is crucial for reproductive studies in non-human primates.
    • Existing methods for cycle staging can be time-consuming or require specialized equipment.
    • Rhesus monkeys are widely used models for human reproductive research.

    Purpose of the Study:

    • To develop and validate a simple, rapid, and cost-effective method for classifying menstrual cycle phases in adult rhesus monkeys.
    • To assess the accuracy of cellular identification in vaginal smears stained with red ink for cycle staging.

    Main Methods:

    • Adult rhesus monkeys (n=37 cycles) were studied.
    • Vaginal smears were collected and stained with red ink.

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  • Cellular morphology in stained smears was analyzed to classify each phase of the menstrual cycle.

    • Main Results:

    • The red ink staining technique allowed for accurate classification of all menstrual cycle phases.
    • The method proved to be simple, quick, and inexpensive.
    • Well-stained slides were produced within seconds, facilitating rapid analysis.

    Conclusions:

    • Cellular identification of red ink-stained vaginal smears is a reliable and efficient method for staging the menstrual cycle in rhesus monkeys.
    • This technique offers a practical alternative for reproductive research in non-human primates.