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Preparation of stable intermediate-purity factor VIII concentrate with a note on high-purity factor VIII
Vox Sanguinis
|January 1, 1979
Summary
This study details a method for producing a factor VIII concentrate from cryoprecipitate. The process involves rapid thawing, washing, adsorption, and filtration, yielding a potent concentrate suitable for further purification.
Area of Science:
- Biochemistry
- Hematology
- Biotechnology
Background:
- Cryoprecipitate is a blood product rich in various clotting factors.
- Efficient isolation of specific clotting factors like factor VIII is crucial for treating bleeding disorders.
Purpose of the Study:
- To develop and describe a method for preparing a crude factor VIII concentrate from cryoprecipitate.
- To assess the yield and composition of the factor VIII concentrate produced.
Main Methods:
- Cryoprecipitate from multiple donors was rapidly thawed and washed.
- The material underwent adsorption with aluminum hydroxide and kaolin, followed by centrifugation.
- The supernatant was diluted, passed through Celite, filtered, and lyophilized.
Main Results:
- The process yielded a factor VIII concentrate with a typical composition of 15 U/ml factor VII and 40 mg/ml protein.
- The overall yield was 300 U/l of starting plasma.
- The crude concentrate proved suitable for subsequent high-purity factor VIII preparation using chromatography.
Conclusions:
- A robust method for preparing factor VIII concentrate from cryoprecipitate has been established.
- The concentrate is a viable starting material for producing highly purified factor VIII.
- This method offers potential for improved hemophilia treatment strategies.