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Complement-fixing antigens in Burkitt lymphoma cell lines.

K J McCormick, W A Stenback, D M Mumford

    Infection and Immunity
    |July 1, 1971
    PubMed
    Summary
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    Researchers studied complement-fixing (CF) activity in Burkitt lymphoma cells. Most sera showed CF activity, correlating with the presence of a herpes-like virus, suggesting a link between this virus and the cancer.

    Area of Science:

    • Virology
    • Immunology
    • Oncology

    Background:

    • Burkitt lymphoma is a B-cell cancer associated with Epstein-Barr virus.
    • Complement fixation (CF) is an immunological assay used to detect antibodies or antigens.
    • Herpes-like viruses have been observed in Burkitt lymphoma cell lines.

    Purpose of the Study:

    • To determine the complement-fixing (CF) activity of antigens from cultured Burkitt lymphoma cells.
    • To investigate the relationship between CF activity, herpes-like virus presence, and immunofluorescence (IF) antigen in these cells.

    Main Methods:

    • Cultured Burkitt lymphoma cells were used as a source of antigens.
    • Normal American sera served as the antibody source for complement fixation tests.
    • Electron microscopy was used to detect herpes-like viruses.

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  • Henle's immunofluorescence (IF) test was employed to detect another antigen.

    • Main Results:

    • Approximately 75% of tested sera exhibited complement-fixing activity against the cultured Burkitt lymphoma cell lines.
    • The presence of a herpes-like virus, detectable by electron microscopy, was confirmed in these cell lines.
    • The level of CF antigen varied between cell lines but did not correlate with the number of cells producing IF antigen.
    • Sera positive in the IF test also contained CF antibodies against crude cell extracts.

    Conclusions:

    • Cultured Burkitt lymphoma cells possess complement-fixing antigens.
    • The presence of a herpes-like virus in these cells is associated with complement-fixing activity.
    • CF antibody responses are linked to the presence of IF antigens in Burkitt lymphoma cells.