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Effects of intrarenal inoculation of Staphylococcus aureus on mice
Abstract:
The extreme susceptibility of the mouse kidney to infection with Staphylococcus aureus was confirmed by direct intrarenal (i.r.) inoculation with this organism. By applying Poisson's distribution formula to the results from infection of mice with small inocula (as few as one coccus or less per kidney), it was estimated that even three organisms could multiply in situ to produce abscess lesions, if the organisms were inoculated directly into the kidneys. This susceptibility of the mouse kidney for staphylococcal infection was not uniformly manifested with every strain, but correlated well with the virulence found through intravenous infection of the strains tested. The i.r. inoculation method by which infection of mice was easily established, using a small inoculum of S. aureus, similar to the amount suspected to occur in natural infections of man, is applicable for the analysis of the mechanisms of staphylococcal infections and any resulting immunity in man. The present paper describes the details and some experimental results obtained by our method.
Insights
Mouse kidneys are highly susceptible to Staphylococcus aureus infections. Even a few bacteria, when directly inoculated, can cause kidney abscesses, offering a model for human infection studies.
Area of Science:
- Microbiology
- Immunology
- Nephrology
Background:
- The mouse kidney exhibits extreme susceptibility to Staphylococcus aureus (S. aureus) infection.
- Understanding the mechanisms of S. aureus kidney infections is crucial for developing effective treatments.
Purpose of the Study:
- To confirm the high susceptibility of the mouse kidney to S. aureus infection.
- To establish a reliable method for inducing and studying S. aureus kidney infections in mice.
- To correlate strain virulence with infection outcomes in the mouse kidney model.
Main Methods:
- Direct intrarenal (i.r.) inoculation of mice with varying doses of S. aureus.
- Application of Poisson's distribution formula to quantify bacterial inoculum and predict lesion formation.
- Comparison of infection outcomes across different S. aureus strains, correlating with previously determined virulence.
Main Results:
- Direct i.r. inoculation readily established S. aureus kidney infections in mice.
- Mathematical modeling suggested that as few as three S. aureus organisms could initiate abscesses in the kidney.
- Kidney infection susceptibility varied among S. aureus strains, correlating with their overall virulence.
Conclusions:
- The mouse kidney serves as a susceptible model for S. aureus infection.
- The i.r. inoculation method is effective for studying S. aureus pathogenesis and immunity.
- This model mimics potential natural human infections, aiding in the analysis of infection mechanisms and immunity.