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Updated: Aug 16, 2026

Immuno-fluorescence Assay of Leptospiral Surface-exposed Proteins
Published on: July 1, 2011
Clinical application of immunofluorescence. 3. Identification of Lancefield group B streptococci
Abstract:
The identification of Lancefield group B streptococci with immunofluorescence was studied after significant differences between the precipitin and immunofluorescent results were noted. It was found that strains of group B streptococci, unlike groups A, C, and G streptococci, do not reliably react with a fluorescein isothiocyanate-labeled conjugate that contains antibodies mainly against C polysaccharide. It was assumed that the difficulty probably resulted from a blocking of the antigen-antibody reaction between the group antigen and its antibody by the S antigen located in the capsule. On the basis of this assumption, a conjugate was prepared that contained antibodies against the various S antigens of group B and it was found to be effective.
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