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Pyocine typing of clinical strains of Pseudomonas aeruginosa
Abstract:
A total of 954 clinical isolates of Pseudomonas aeruginosa were typed by their ability to produce pyocines. The strains of Pseudomonas were isolated from urines, bloods, sputa, stools, and miscellaneous infectious exudates or tissue of patients of the Mayo Clinic and four associated hospitals. About 80% of the typable strains could be grouped into three major pyocine types: A (30.9%), B (34.8%), and D (14.1%). These large groups could be divided into subtypes by using additional indicator strains. There was no significant difference in the distribution of types by either institutional or specimen source, except that urine specimens yielded the highest percentage of one type. By this procedure, 93% of all isolates could be typed. Repeated typing of serially transferred strains indicated that the procedure has a high degree of reliability. Several strains exhibited extreme fluctuation in inhibition pattern. The procedure is a simple and reliable method to monitor the patterns of nosocomial infections due to P. aeruginosa.
Insights
This study shows pyocine typing reliably identifies Pseudomonas aeruginosa strains, with types A, B, and D dominating. This method aids in monitoring hospital-acquired infections caused by this bacterium.
Area of Science:
- Clinical microbiology
- Infectious diseases
- Bacteriology
Background:
- Pseudomonas aeruginosa is a significant opportunistic pathogen.
- Effective monitoring of nosocomial infections is crucial.
Purpose of the Study:
- To evaluate pyocine typing as a method for characterizing Pseudomonas aeruginosa clinical isolates.
- To determine the prevalence of different pyocine types in clinical settings.
Main Methods:
- Typing of 954 clinical isolates of Pseudomonas aeruginosa based on pyocine production.
- Isolation of strains from various human specimens (urine, blood, sputum, stool, exudates).
- Utilizing indicator strains for detailed subtyping.
Main Results:
- 93% of isolates were successfully typed.
- Three major pyocine types (A, B, D) accounted for approximately 80% of typable strains.
- No significant variation in type distribution by institution or specimen source, with urine showing a slight difference.
Conclusions:
- Pyocine typing is a simple, reliable method for monitoring Pseudomonas aeruginosa.
- The established typing scheme effectively characterizes clinical isolates.
- This technique can aid in tracking nosocomial infections caused by P. aeruginosa.