Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Antiserum induced myelination inhibition in vitro without complement.

S H Dorfman, J M Fry, D H Silberberg

    Brain Research
    |November 9, 1979
    PubMed
    Summary

    Antibodies targeting central nervous system (CNS) tissue or galactocerebroside blocked neonatal rat cerebellum myelination. Myelination resumed after antibody removal, suggesting a non-cytotoxic mechanism.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Conservatism in Gun-Shot Wounds of the Abdomen.

    Texas medical journal (Austin, Tex.)·2023
    Same author

    Adult human brain expresses four different molecular forms of neural cell adhesion molecules.

    Neurochemistry international·2010
    Same author

    Immunologic characterization of oligodendroglia.

    Neuroscience letters·2009
    Same author

    Disease modifying therapies in multiple sclerosis: report of the Therapeutics and Technology Assessment Subcommittee of the American Academy of Neurology and the MS Council for Clinical Practice Guidelines [RETIRED].

    Neurology·2002
    Same author

    Long-term follow-up on restless legs syndrome patients treated with opioids.

    Movement disorders : official journal of the Movement Disorder Society·2001
    Same author

    A double-blind, placebo-controlled trial of extracorporeal photopheresis in chronic progressive multiple sclerosis.

    Multiple sclerosis (Houndmills, Basingstoke, England)·1999

    Area of Science:

    • Neuroscience
    • Immunology
    • Developmental Biology

    Background:

    • Myelination is crucial for proper nervous system function.
    • The role of the immune system, specifically complement, in regulating myelination is not fully understood.

    Purpose of the Study:

    • To investigate the mechanism by which anti-central nervous system (CNS) tissue antiserum inhibits primary myelination in neonatal rat cerebellum cultures.

    Main Methods:

    • Neonatal rat cerebellum cultures were exposed to antiserum against whole spinal cord or galactocerebroside.
    • Complement activity was assessed by using fresh or heat-inactivated guinea pig serum.
    • Myelination was monitored after antiserum removal.

    Main Results:

    • Antiserum to whole spinal cord or galactocerebroside inhibited myelination, irrespective of complement activity.
    • Myelination recommenced upon removal of the antisera from the culture medium.

    Conclusions:

    • The inhibition of primary myelination by anti-CNS tissue antiserum is not mediated by complement-dependent cytotoxicity against oligodendrocytes.
    • Alternative non-cytotoxic mechanisms are likely involved in antibody-mediated inhibition of myelination.

    Related Experiment Videos