Separation and assay of iron proteins in needle biopsy specimens of human liver

A micro method has been developed for the separation of the principal classes of iron proteins in needle biopsy specimens of human liver. The iron content of the fractions was determined by automated flameless atomic absorption spectrophotometry in a one step procedure. The levels of total iron, transferrin-, ferritin-, haemprotein- and haemosiderin-iron are reported for control tissue.