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Related Experiment Videos

Quantitative high-performance liquid-chromatographic method for determining disopyramide (Norpace) in serum.

L A Broussard, C S Frings

    Clinical Toxicology
    |January 1, 1979
    PubMed
    Summary

    A new high-performance liquid-chromatographic method accurately measures disopyramide in serum. This validated assay offers high sensitivity and precision for clinical and research applications.

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    Area of Science:

    • Analytical Chemistry
    • Clinical Chemistry
    • Pharmacokinetics

    Background:

    • Disopyramide is an antiarrhythmic drug requiring therapeutic drug monitoring.
    • Accurate quantification of disopyramide in biological matrices is crucial for patient management.

    Purpose of the Study:

    • To develop and validate a sensitive and specific high-performance liquid-chromatographic (HPLC) method for disopyramide serum measurement.

    Main Methods:

    • Serum samples (0.5 ml) were extracted with chloroform containing p-chlorodisopyramide as an internal standard.
    • Separation was performed on a reversed-phase octadecylsilyl column at room temperature.
    • Detection was achieved at 254 nm.

    Main Results:

    • The method demonstrated sensitivity down to 0.2 mg/L with linearity up to 10 mg/L.

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  • Within-day and day-to-day precision (CV) were below 10% at various concentrations.
  • Average recovery was 98%, with minimal interference from over 85 other drugs.

    • Conclusions:

    • This HPLC method provides a reliable, sensitive, and specific means for quantifying disopyramide in serum.
    • The assay is suitable for routine clinical laboratory use and pharmacokinetic studies.