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Related Experiment Videos

Protein synthesis in imbibing wheat embryos.

A C Cuming, B G Lane

    European Journal of Biochemistry
    |September 1, 1979
    PubMed
    Summary

    Wheat embryo protein synthesis during germination was studied using cell-free systems. Early imbibition produces ribosomal proteins, with changes occurring after 40 minutes.

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    Area of Science:

    • Molecular Biology
    • Plant Science
    • Biochemistry

    Background:

    • Wheat embryo protein synthesis is crucial for germination and early development.
    • Understanding gene expression during seed imbibition provides insights into developmental transitions.

    Purpose of the Study:

    • To compare polypeptide synthesis in imbibing wheat embryos with cell-free systems.
    • To investigate changes in protein synthesis patterns during wheat seed germination.

    Main Methods:

    • Wheat embryos and cell-free extracts (rabbit reticulocyte lysate, wheat germ extract) were used.
    • Polypeptides were synthesized using [35S]methionine labeling.
    • Protein separation and analysis were performed using one-dimensional and two-dimensional electrophoresis and fluorography.

    Main Results:

    • Cell-free systems programmed with dry wheat embryo RNA produced polypeptides similar to early imbibition.
    • Significant changes in polypeptide profiles were observed after 40 minutes of imbibition.
    • Low-molecular-weight and basic polypeptides decreased in proportion during later imbibition.
    • Ribosomal proteins were prominent during early imbibition and in cell-free synthesis.

    Conclusions:

    • Cell-free systems accurately reflect early protein synthesis in imbibing wheat embryos.
    • Germination involves dynamic shifts in protein synthesis, with a decrease in certain polypeptide types over time.
    • Ribosomal protein synthesis is a key early event during wheat embryo germination.

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