Jove
Visualize
Contact Us

Related Experiment Videos

The Feulgen reaction after glutaraldehyde fixation.

F H Kasten, R Lala

    Stain Technology
    |May 1, 1975
    PubMed
    Summary

    This study presents a new method for DNA staining using the Feulgen reaction on glutaraldehyde-fixed samples. The technique effectively blocks aldehydes, ensuring accurate DNA content measurement in cells and tissues.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    A Critical Understanding of Inclusion in Oral Microbiome Research through the Lens of Racial Capitalism.

    Community dental health·2024
    Same author

    The Case for Decolonising the Dental Curricula in the UK.

    Community dental health·2022
    Same author

    A Critical Analysis of Underrepresentation of Racialised Minorities in the UK Dental Workforce.

    Community dental health·2021
    Same author

    The Relevance of Power in Dentistry.

    JDR clinical and translational research·2021
    Same author

    The use of the NICE ten step model to conduct an oral health needs assessment in South Yorkshire and Bassetlaw.

    Community dental health·2018
    Same author

    The dental public health implications of cosmetic dentistry: a scoping review of the literature.

    Community dental health·2017
    JoVE
    x logofacebook logolinkedin logoyoutube logo
    ABOUT JoVE
    OverviewLeadershipBlogJoVE Help Center
    AUTHORS
    Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
    LIBRARIANS
    TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
    RESEARCH
    JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
    EDUCATION
    JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
    Terms & Conditions of Use
    Privacy Policy
    Policies

    Area of Science:

    • Biochemistry
    • Cell Biology
    • Histology

    Background:

    • Glutaraldehyde fixation is common for biological samples.
    • Unreacted aldehydes can interfere with staining techniques like the Feulgen reaction.
    • Accurate DNA quantification is crucial in various biological studies.

    Purpose of the Study:

    • To develop a reliable method for performing the Feulgen reaction on glutaraldehyde-fixed cells and tissues.
    • To ensure that aldehyde blockade does not affect DNA content measurements.
    • To optimize the Feulgen reaction protocol for preserved samples.

    Main Methods:

    • Samples were fixed using glutaraldehyde.
    • Free aldehydes were blocked using sodium borohydride (NaBH4).
    • The Feulgen reaction was performed, involving acid hydrolysis and Schiff's reagent staining.
    • Cytophotometry was used to measure nuclear DNA content.

    Main Results:

    • The sodium borohydride blockade effectively neutralized free aldehydes.
    • Controls confirmed the blockade's completeness and irreversibility.
    • Cytophotometric analysis demonstrated that the DNA content per nucleus remained unchanged after the blockade procedure.

    Conclusions:

    • The described technique enables accurate Feulgen staining for DNA in glutaraldehyde-fixed specimens.
    • Aldehyde blockade is a critical step for reliable DNA quantification.
    • This method preserves the integrity of nuclear DNA during the staining process.

    Related Experiment Videos