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Related Concept Videos

Solution Composition During Acid/Base Titrations01:17

Solution Composition During Acid/Base Titrations

The titration of a weak acid with a strong base results in the formation of water and the conjugate base of the acid. For instance, titrating acetic acid with sodium hydroxide leads to the formation of water and sodium acetate. A solution of acetic acid and sodium acetate constitutes a buffer whose relative concentration at different stages of the titration is indicated by the α values, which represent percentages of the weak acid and its conjugate base.
The α0 and α1 values represent the...
Titration of Polyprotic Base with a Strong Acid01:18

Titration of Polyprotic Base with a Strong Acid

The titration of a polyprotic base such as sodium carbonate with a strong acid such as hydrochloric acid results in two equivalence points on the titration curve. At the first equivalence point, the carbonate ions in the base are completely converted to bicarbonate ions. The second equivalence point corresponds to the complete conversion of bicarbonate ions to carbonic acid, which dissociates into carbon dioxide and water. The region before the first equivalence point corresponds to the...
Voltammetric Techniques: Linear-Scan (E vs Time)01:12

Voltammetric Techniques: Linear-Scan (E vs Time)

Polarography is a classical voltammetric technique used to analyze electrochemical reactions. This method applies a linear potential sweep to a dropping mercury electrode (DME), and the resulting current is measured. A dropping mercury electrode is commonly used as the working electrode in polarography. It consists of a capillary tube filled with mercury, where the tiny droplet forms at the tip. This droplet continuously drops from the capillary, creating a new electrode surface for each...
EDTA: Indirect and Alkalimetric Titration01:23

EDTA: Indirect and Alkalimetric Titration

Unlike direct titration, back-titration, and displacement titration, indirect titration is an EDTA titration method for quantifying anions. In the indirect titration method, anions are precipitated as their insoluble salts with excess metal ions. The filtrate containing the excess metal ions is directly titrated with standard EDTA until the endpoint is achieved. Another approach involves extracting the metal ion and back-titrating with standard EDTA to obtain the endpoint. In this way, the...
Diagnosing Acidosis and Alkalosis01:24

Diagnosing Acidosis and Alkalosis

Diagnosing acid-base imbalances involves systematically analyzing arterial blood samples, focusing on three key measurements: pH, bicarbonate (HCO3−) concentration, and carbon dioxide partial pressure (PCO2). This analysis follows a four-step process that helps identify the imbalance's underlying cause and nature.
First, the pH level is assessed to determine whether the blood pH is normal (7.35–7.45), low (acidosis), or high (alkalosis).
Next, the PCO2  and HCO3−  values are examined to...
Titration of a Weak Acid with a Strong Base01:30

Titration of a Weak Acid with a Strong Base

In titrating a weak acid with a strong base, different calculation methods are applied at various stages. Initially, the pH of a weak acid like acetic acid is calculated using its dissociation constant (Ka) and an ICE table. Upon addition of a strong base such as sodium hydroxide, a buffer forms, and its pH is determined using the Henderson-Hasselbalch equation. As more base is added and the titration reaches the halfway point, the pH becomes equal to the pKa of the acid, indicating equal...

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Related Experiment Video

Updated: May 12, 2026

Measurement and Analysis of Extracellular Acid Production to Determine Glycolytic Rate
06:47

Measurement and Analysis of Extracellular Acid Production to Determine Glycolytic Rate

Published on: December 12, 2015

A single scan technique for estimating acid output.

T V Taylor, S Holt, G P McLoughlin

    Gastroenterology
    |December 1, 1979
    PubMed
    Summary
    This summary is machine-generated.

    A new 99mTc scan technique accurately measures gastric acid output. This rapid, minimally invasive method offers a convenient alternative to traditional gastric aspiration for patients.

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    Using Capillary Electrophoresis to Quantify Organic Acids from Plant Tissue: A Test Case Examining Coffea arabica Seeds
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    Published on: November 12, 2016

    Estimation of Nephron Number in Whole Kidney using the Acid Maceration Method
    08:15

    Estimation of Nephron Number in Whole Kidney using the Acid Maceration Method

    Published on: May 22, 2019

    Related Experiment Videos

    Last Updated: May 12, 2026

    Measurement and Analysis of Extracellular Acid Production to Determine Glycolytic Rate
    06:47

    Measurement and Analysis of Extracellular Acid Production to Determine Glycolytic Rate

    Published on: December 12, 2015

    Using Capillary Electrophoresis to Quantify Organic Acids from Plant Tissue: A Test Case Examining Coffea arabica Seeds
    10:13

    Using Capillary Electrophoresis to Quantify Organic Acids from Plant Tissue: A Test Case Examining Coffea arabica Seeds

    Published on: November 12, 2016

    Estimation of Nephron Number in Whole Kidney using the Acid Maceration Method
    08:15

    Estimation of Nephron Number in Whole Kidney using the Acid Maceration Method

    Published on: May 22, 2019

    Area of Science:

    • Nuclear Medicine
    • Gastroenterology
    • Diagnostic Imaging

    Background:

    • Gastric acid output measurement is crucial for diagnosing and managing gastrointestinal disorders.
    • Conventional methods like gastric aspiration are invasive, uncomfortable, and time-consuming.
    • There is a need for a simpler, non-invasive technique to assess gastric acid production.

    Purpose of the Study:

    • To develop and validate a single-scan nuclear medicine technique for measuring gastric acid output.
    • To compare the accuracy of the new method with conventional gastric acid measurement techniques.
    • To evaluate the feasibility and patient comfort of the novel scanning procedure.

    Main Methods:

    • A single-scan technique using technetium-99m (99mTc) was developed.
    • Patients underwent an overnight fast, followed by pentagastrin administration and intravenous 99mTc injection.
    • Abdominal scans were performed using a double-headed scanner, and isotope accumulation in the stomach was quantified.

    Main Results:

    • Intragastric 99mTc accumulation showed a strong positive correlation with conventionally measured gastric acid output (r = 0.87, P < 0.0001).
    • The developed scan technique is rapid and requires minimal patient inconvenience.
    • The radiation dose administered to patients is small.

    Conclusions:

    • The 99mTc single-scan technique provides a reliable and accurate method for measuring pentagastrin-stimulated gastric acid output.
    • This nuclear medicine approach offers significant advantages over invasive gastric aspiration methods.
    • The technique is well-tolerated by patients and suitable for routine clinical use.