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Absorption of factor VIII by aluminium hydroxide
Haemostasis
|January 1, 1979
Summary
Aluminium hydroxide effectively absorbs factor VIII (clotting protein) at low concentrations, with higher absorption rates as protein levels decrease. This method shows potential for factor VIII purification but requires further optimization for yield.
Area of Science:
- Biochemistry
- Hematology
- Protein Chemistry
Background:
- Factor VIII (FVIII) is crucial for blood coagulation.
- Aluminium hydroxide is used in some bioprocessing applications.
- Understanding FVIII interaction with adsorbents is key for purification.
Purpose of the Study:
- To investigate the absorption of Factor VIII by aluminium hydroxide.
- To determine the effect of protein concentration on FVIII absorption.
- To assess the selectivity and yield of FVIII recovery.
Main Methods:
- Studied FVIII absorption from various sources (fractions, concentrates, plasma) using aluminium hydroxide.
- Varied initial protein concentrations to assess absorption efficiency.
- Analyzed absorbed and eluted proteins for Factor VIII antigen (VIII R:Ag) and activity (VIII:C).
Main Results:
- Significant FVIII absorption occurred at low protein concentrations, increasing as concentration decreased.
- Factor VIII R:Ag and VIII:C were absorbed in parallel from less pure samples.
- High-purity concentrate absorption selectively removed lower molecular weight FVIII forms.
- Elution yielded poor VIII R:Ag recovery and undetectable VIII:C.
- Plasma absorption showed no VIII R:Ag loss but a ~10% decrease in VIII:C.
Conclusions:
- Aluminium hydroxide can absorb Factor VIII, particularly at low concentrations.
- The process shows potential for FVIII purification, but yield and selectivity vary with sample purity.
- Further research is needed to optimize elution for better recovery of functional Factor VIII.