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Related Experiment Videos

Radiometric method for detection of bacteremia.

J A Washington, P K Yu

    Applied Microbiology
    |July 1, 1971
    PubMed
    Summary

    This study found that detecting carbon dioxide (CO2) from bacterial growth in blood cultures was not effective. The radiometric method, measuring CO2, did not offer earlier detection of bacteremia compared to standard methods.

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    Area of Science:

    • Microbiology
    • Clinical Diagnostics
    • Radiochemistry

    Background:

    • Early detection of bacteremia is crucial for effective patient treatment.
    • Radiometric methods, detecting radiolabeled carbon dioxide (CO2), have been explored for bacterial growth monitoring.
    • Assessing the utility of (14)CO(2) production as a reliable indicator of bacterial proliferation in blood cultures is essential.

    Purpose of the Study:

    • To evaluate the efficacy of detecting (14)CO(2) production as an index of bacterial growth in simulated and patient blood cultures.
    • To determine if the radiometric method offers earlier detection of bacteremia compared to conventional methods.

    Main Methods:

    • Simulated blood cultures were inoculated with varying concentrations of bacteria (4 to 4,250 colony-forming units).
    • The production of (14)CO(2) was monitored as an indicator of bacterial growth.
    • A limited trial involved comparing the radiometric method with routine broth cultures using patients' blood samples.

    Main Results:

    • In 59 experiments with simulated blood cultures, (14)CO(2) was not detectable within 6 hours post-inoculation, irrespective of inoculum size.
    • The radiometric method did not yield earlier detection of bacteremia in patient blood cultures compared to standard broth cultures.

    Conclusions:

    • The detection of (14)CO(2) is not a sensitive or timely index for bacterial growth in blood cultures.
    • The radiometric method, as evaluated, does not provide a significant advantage over routine methods for the early diagnosis of bacteremia.

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