Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Enzyme purification by electrodecantation.

B G Winchester, M Caffrey, D Robinson

    The Biochemical Journal
    |January 1, 1971
    PubMed
    Summary
    This summary is machine-generated.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Animal medical genetics: a historical perspective on more than 50 years of research into genetic disorders of animals at Massey University.

    New Zealand veterinary journal·2021
    Same author

    Enzyme analysis for Pompe disease in leukocytes; superior results with natural substrate compared with artificial substrates.

    Journal of inherited metabolic disease·2009
    Same author

    Molecular defects in Sanfilippo syndrome type B (mucopolysaccharidosis IIIB).

    Journal of inherited metabolic disease·2005
    Same author

    Clinical and molecular features of three patients with congenital disorders of glycosylation type Ih (CDG-Ih) (ALG8 deficiency).

    Journal of medical genetics·2004
    Same author

    Mass spectrometric analysis of glycans in elucidating the pathogenesis of CDG type IIx .

    Journal of inherited metabolic disease·2003
    Same author

    Sanfilippo syndrome type D: identification of the first mutation in the N-acetylglucosamine-6-sulphatase gene.

    Journal of medical genetics·2003
    Same journal

    Nanobodies against Plasmodium adhesins that block receptor engagement and malaria parasite invasion.

    The Biochemical journal·2026
    Same journal

    Persistence without turnover: the RhoG G12E mutant highlights the role of nucleotide cycling in RhoG signaling.

    The Biochemical journal·2026
    Same journal

    Alternative Splicing of Rice Chloroplastic CuZn Superoxide Dismutase, OsCSD2: Impact on expression and protein characteristics.

    The Biochemical journal·2026
    Same journal

    Difference and similarity between the ubiquitous secretory pathway Ca2+-ATPases, SERCA2b, and SPCA1a.

    The Biochemical journal·2026
    Same journal

    A molecular perspective on dimethylarginine dimethylaminohydrolases structure and function.

    The Biochemical journal·2026
    Same journal

    Proteolytic coordination of the OXPHOS Life Cycle.

    The Biochemical journal·2026
    See all related articles

    Electrodecantation effectively fractionates enzymes like beta-d-glucosidase and N-acetylglucosaminidase, achieving significant purification. However, this method is less suitable for concentrating very dilute enzyme solutions due to activity loss.

    Area of Science:

    • Biochemistry
    • Enzyme Purification
    • Separation Techniques

    Background:

    • Enzyme purification is crucial for biochemical research and applications.
    • Traditional methods can be time-consuming and may lead to enzyme denaturation.
    • Novel techniques are needed for efficient and scalable enzyme preparation.

    Purpose of the Study:

    • To evaluate electrodecantation as a method for initial enzyme fractionation.
    • To determine the efficacy of electrodecantation for purifying specific enzymes.
    • To assess the limitations and potential of electrodecantation in laboratory-scale enzyme preparation.

    Main Methods:

    • Electrodecantation was applied to crude extracts of pig kidney beta-d-glucosidase and sheep testicular N-acetylglucosaminidase.

    Related Experiment Videos

  • Isoelectric focusing was used to determine the isoelectric points of the enzymes.
  • Purification was performed at the determined isoelectric points using electrodecantation.

    • Main Results:

    • A six- to ten-fold increase in specific activity was achieved for both enzymes after a single electrodecantation cycle.
    • Good recoveries of enzyme activity were obtained.
    • Further purification of a Bacillus subtilis protease preparation was also demonstrated.

    Conclusions:

    • Electrodecantation is a viable technique for the initial fractionation and purification of enzymes.
    • The method shows promise for laboratory-scale enzyme preparation, yielding significant activity increases.
    • Concentrating dilute enzyme solutions using electrodecantation resulted in substantial activity loss, highlighting a key limitation.