Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Externally suppressible proline quadruplet ccc U.

J Yourno, T Kohno

    Science (New York, N.Y.)
    |February 11, 1972
    PubMed
    Summary

    Specific mutations can be corrected by a single suppressor, restoring normal protein function. This suggests a proline transfer RNA may be responsible for correcting these frameshift mutations.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Growth suppression of non-small cell lung carcinoma cells by the introduction of the p16(INK4A) gene.

    International journal of oncology·2011
    Same author

    Histone acetylation by CBP and p300 at double-strand break sites facilitates SWI/SNF chromatin remodeling and the recruitment of non-homologous end joining factors.

    Oncogene·2011
    Same author

    Optical system of the Infrared Telescope in Space.

    Applied optics·2010
    Same author

    High precision optical surface sensor.

    Applied optics·2010
    Same author

    [Which tube is better for esophagectomy?].

    Kyobu geka. The Japanese journal of thoracic surgery·2009
    Same author

    Spontaneous hyperactivity in mutant mice lacking the NMDA receptor GluRepsilon1 subunit is aggravated during exposure to 0.1 MAC sevoflurane and is preserved after emergence from sevoflurane anaesthesia.

    European journal of anaesthesiology·2008

    Area of Science:

    • Molecular Biology
    • Genetics
    • Biochemistry

    Background:

    • Frameshift mutations alter the reading frame of genetic code, often leading to non-functional proteins.
    • External suppressors can sometimes partially restore protein function by interacting with mutated sequences.

    Purpose of the Study:

    • To investigate the mechanism by which a single external suppressor corrects multiple frameshift mutations.
    • To identify the specific genetic sequence and the nature of the suppressor agent involved.

    Main Methods:

    • Analysis of (+1) frameshift mutations at different genetic loci.
    • Protein characterization (electrophoretic mobility, heat stability, amino acid sequencing).
    • Identification of common messenger RNA sequences at mutation sites.

    Main Results:

    • A single external suppressor restored functional protein from three distinct frameshift mutations.
    • The restored proteins exhibited normal electrophoretic mobility and heat stability.
    • Wildtype amino acid sequence was confirmed for one restored protein.
    • A common messenger RNA quadruplet, CCCU, was identified at all three mutation sites.
    • This sequence appears to be translated as proline by the suppressor.

    Conclusions:

    • A proline transfer RNA with a quadruplet anticodon, or a similar functional molecule, is a likely candidate for the suppressor agent.
    • This suppressor specifically recognizes and corrects frameshift mutations associated with the CCCU sequence.
    • The findings provide insight into suppressor tRNA mechanisms and frameshift mutation repair.

    Related Experiment Videos