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Related Experiment Videos

Cell separation with staphylococcal protein A immunoadsorbent surfaces.

P G Bundesen, J Gordon

    Journal of Immunological Methods
    |January 1, 1979
    PubMed
    Summary
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    A novel indirect cell separation method uses protein A and specific antisera for efficient cell isolation. This technique ensures high specificity, cell viability, and recovery, applicable across various antibody classes and species.

    Area of Science:

    • Immunology
    • Biotechnology
    • Cell Biology

    Background:

    • Cell separation is crucial for various biological and medical applications.
    • Existing methods may have limitations in specificity, efficiency, or cell viability.

    Purpose of the Study:

    • To develop an "indirect" cell separation technique using protein A.
    • To achieve efficient and specific cell separation with high cell viability and recovery.

    Main Methods:

    • Cells were first reacted with specific antisera.
    • Separation was performed on antibody/protein A immunoadsorbent surfaces.
    • The antibody coupled to protein A targeted the initial antisera bound to cells.

    Main Results:

    • The technique demonstrated excellent specificity, cell viability, and total cell recoveries.

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  • It requires only small volumes of specific antisera for separation.
  • The method is scalable for both small and large-scale applications.

    • Conclusions:

    • The developed indirect cell separation technique is highly effective and versatile.
    • It offers significant advantages over existing cell separation methods.
    • The technique has the potential for broad application across different antibody classes and species.