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Urinary inorganic phosphorus determinations.

D H Jung, A C Parekh

    Journal of Clinical Pathology
    |March 1, 1972
    PubMed
    Summary
    This summary is machine-generated.

    The Parekh-Jung method accurately determines inorganic phosphorus in urine, showing good precision and recovery. This study validates its use for urine analysis, comparing it to other methods.

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    Area of Science:

    • Clinical Chemistry
    • Analytical Chemistry
    • Biochemistry

    Background:

    • Inorganic phosphorus is a crucial analyte in both serum and urine.
    • Accurate determination of urinary phosphorus is essential for diagnosing metabolic disorders.
    • Existing methods for urinary phosphorus analysis may have limitations.

    Purpose of the Study:

    • To evaluate the applicability and performance of the Parekh-Jung method for inorganic phosphorus determination in urine.
    • To assess the accuracy and precision of this method in a urine matrix.
    • To compare the Parekh-Jung method with other established techniques for urinary phosphorus analysis.

    Main Methods:

    • Adaptation of the Parekh-Jung method, originally for serum, to urine samples.
    • Quantification of inorganic phosphorus using spectrophotometry.

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  • Assessment of accuracy through percentage recovery of spiked phosphorus.
  • Evaluation of between-batch precision through replicate analyses of a urine sample.

    • Main Results:

    • The Parekh-Jung method demonstrated good accuracy for urinary inorganic phosphorus.
    • Mean percentage recovery of added phosphorus was 100.2%.
    • Between-batch precision showed a mean value of 46.71 mg/100 ml with a standard deviation of +/- 0.76.

    Conclusions:

    • The Parekh-Jung method is a reliable and accurate technique for the determination of inorganic phosphorus in urine.
    • Its performance characteristics suggest it is a viable alternative to existing methods.
    • Further discussion on its relative usefulness compared to other methods is provided.