Related Experiment Videos
Long-term cranial mesenteric vein cannulation in the rat
Laboratory Animal Science
|June 1, 1979
Summary
A novel catheter design using polyethylene and silicone rubber maintained patency in 85% of rat cranial mesenteric veins for 3 weeks, enabling repeated blood sampling and injections.
Area of Science:
- Veterinary Medicine
- Surgical Techniques
- Animal Models
Background:
- Vascular access in animal research is crucial for drug delivery and sample collection.
- Maintaining catheter patency over extended periods is a significant challenge in rodent models.
Purpose of the Study:
- To evaluate the patency and reliability of a novel polyethylene-tipped silicone rubber catheter for repeated vascular access in rats.
- To assess the suitability of this catheter for serial blood withdrawal and injections in a rodent model.
Main Methods:
- A polyethylene-tipped silicone rubber catheter was surgically implanted into the cranial mesenteric vein of rats.
- The catheter's patency was assessed over a 3-week period.
- Injections and blood withdrawals were performed an average of seven times per animal.
Main Results:
- The catheter demonstrated high patency, with 17 out of 20 (85%) remaining functional for the entire 3-week study duration.
- The catheter material (polyethylene tip, silicone rubber body) proved durable for repeated procedures.
Conclusions:
- The tested catheter design is a reliable and effective tool for achieving sustained vascular access in rat models.
- This catheter facilitates repeated experimental manipulations, such as blood sampling and injections, without compromising vascular integrity over 3 weeks.