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Valinomycin crystal structure determination by direct methods.

W L Duax, H Hauptman, C M Weeks

    Science (New York, N.Y.)
    |May 26, 1972
    PubMed
    Summary
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    The antibiotic valinomycin

    Area of Science:

    • Biochemistry
    • Structural Biology
    • Medicinal Chemistry

    Background:

    • Valinomycin is a cyclic depsipeptide antibiotic known for its ability to complex with potassium ions.
    • Understanding the precise three-dimensional structure of valinomycin is crucial for elucidating its mechanism of action and for drug design.
    • Previous structural studies have proposed various conformations, but a definitive structure for the uncomplexed form remained elusive.

    Purpose of the Study:

    • To determine the exact three-dimensional conformation of uncomplexed valinomycin.
    • To investigate the intramolecular hydrogen bonding and stereochemistry of the uncomplexed antibiotic.
    • To provide a structural basis for understanding the conformational changes upon potassium ion complexation.

    Main Methods:

    Related Experiment Videos

  • Direct methods were employed for structure determination.
  • A novel technique for enantiomorph discrimination using cosine invariants was utilized.
  • Computational analysis of structural features was performed.

    • Main Results:

    • The precise conformation of uncomplexed valinomycin was determined.
    • The intramolecular hydrogen bonding and isopropyl group stereochemistry were elucidated.
    • The determined structure is distinct from previously proposed models.

    Conclusions:

    • The determined structure of uncomplexed valinomycin is consistent with spectral data in solution.
    • A simple shift in hydrogen bonding can explain the conformational transition to the potassium-ion complexed form.
    • This study provides a refined structural understanding of valinomycin's conformational flexibility and ion-binding mechanism.