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Antigenicity of carcinogen and viral induced sarcomas in inbred and random bred guinea pigs
Abstract:
A tumor specific transplantation antigen (TSTA) has been detected in a methylcholanthrene (MCA) induced guinea pig tumor. It was possible to induce resistance to rechallenge with the tumor by immunization with irradiated cells in CFA. In contrast, the same technique failed to detect TSTA in two viral (Kirsten strain mouse sarcoma virus, Ki-MSV) induced guinea pig tumors; these results are similar to observations made with mouse Ki-MSV-induced tumors. Transplantation studies with these tumors in both inbred and random-bred guinea pigs showed a complexity of growth and rejection patterns. The B alloantigen, a major serologically defined antigen of the guinea pig histocompatibility complex, seemed to play a central role in acting as a guniea pig transplantation antigen. In all cases studied, the absence of B antigens in the recipient led to tumor rejection and anti-B antibody protection.
Insights
Tumor-specific transplantation antigens (TSTA) were found in methylcholanthrene-induced guinea pig tumors but not viral-induced ones. The B alloantigen significantly influenced tumor rejection, with its absence leading to rejection.
Area of Science:
- Oncology
- Immunology
- Transplantation Biology
Background:
- Tumor-specific transplantation antigens (TSTA) are crucial for immune rejection of tumors.
- Methylcholanthrene (MCA)-induced and viral-induced tumors may differ in their antigenicity.
- The role of histocompatibility antigens in tumor transplantation is complex.
Purpose of the Study:
- To investigate the presence of TSTA in MCA-induced and viral-induced guinea pig tumors.
- To analyze the influence of the B alloantigen on tumor transplantation and rejection.
- To understand the mechanisms underlying tumor rejection in guinea pigs.
Main Methods:
- Induction of resistance to tumor rechallenge via immunization with irradiated tumor cells in Complete Freund's Adjuvant (CFA).
- Transplantation studies of MCA-induced and viral-induced (Kirsten strain mouse sarcoma virus, Ki-MSV) guinea pig tumors.
- Analysis of tumor growth and rejection patterns in inbred and random-bred guinea pigs.
- Serological detection of B alloantigens and assessment of anti-B antibody protection.
Main Results:
- TSTA was detected in MCA-induced guinea pig tumors, enabling induced resistance.
- TSTA detection failed in viral-induced (Ki-MSV) guinea pig tumors, mirroring findings in mice.
- Tumor transplantation revealed complex growth and rejection patterns.
- The B alloantigen played a central role; its absence in recipients consistently led to tumor rejection.
- Anti-B antibody administration protected against tumor growth.
Conclusions:
- MCA-induced tumors express TSTA, allowing for immune-mediated resistance.
- Viral-induced tumors in guinea pigs may lack detectable TSTA, similar to mouse models.
- The B alloantigen is a critical factor in guinea pig tumor transplantation, influencing rejection outcomes.
- Targeting the B alloantigen or its associated immune responses could be a strategy for cancer immunotherapy.