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Related Experiment Videos

Changes in CDP-diglyceride:inositol transferase activity during rabbit lung development.

J E Bleasdale, P Wallis, P C MacDonald

    Pediatric Research
    |October 1, 1979
    PubMed
    Summary
    This summary is machine-generated.

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    The activity of CDP-diglyceride:inositol transferase significantly increases in rabbit lungs and liver during development. This rise is due to new enzyme synthesis, not activation of existing enzymes.

    Area of Science:

    • Biochemistry
    • Developmental Biology
    • Enzymology

    Background:

    • CDP-diglyceride:inositol transferase plays a role in cellular lipid metabolism.
    • Understanding enzyme activity during development is crucial for identifying physiological changes.

    Purpose of the Study:

    • To investigate the developmental changes in CDP-diglyceride:inositol transferase activity in rabbit lung tissue.
    • To determine if the observed changes are specific to lung tissue or a general developmental phenomenon.
    • To elucidate the mechanism behind the increase in enzyme activity.

    Main Methods:

    • Enzyme assays were performed on rabbit lung and liver tissues at various gestational and postnatal stages.
    • Microsomal fractions were prepared from fetal and adult rabbit lung tissue.

    Related Experiment Videos

  • Enzyme properties were compared between fetal and adult lung microsomes.

    • Main Results:

    • A significant 8- to 9-fold increase in CDP-diglyceride:inositol transferase specific activity was observed from gestation day 17 to adulthood in rabbit lung tissue.
    • Similar developmental increases in enzyme activity were also noted in rabbit liver tissue, indicating a non-tissue-specific pattern.
    • The kinetic properties of CDP-diglyceride:inositol transferase in microsomes from fetal and adult rabbit lungs were indistinguishable.
    • The data suggest that the increased enzyme activity during development results from the synthesis of new enzyme molecules.

    Conclusions:

    • CDP-diglyceride:inositol transferase activity undergoes substantial upregulation during rabbit development in both lung and liver.
    • The developmental increase in enzyme activity is attributed to de novo synthesis of the enzyme.
    • These findings contribute to the understanding of lipid metabolism regulation during mammalian development.