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An insert for the reorienting gradient rotor for virus extraction from infected plants
Preparative Biochemistry
|January 1, 1979
Summary
A new nylon device for plant virus separation using centrifugation effectively extracts 80-90% of virus particles. This method significantly reduces extraneous materials for clearer virus analysis.
Area of Science:
- Plant pathology
- Biochemistry
- Biotechnology
Background:
- Accurate separation of plant viruses is crucial for disease diagnosis and research.
- Existing methods for virus extraction can be inefficient and yield contaminated samples.
Purpose of the Study:
- To introduce a novel nylon insert device for efficient plant virus separation.
- To evaluate the effectiveness of the device in extracting virus particles from various plant tissue states.
Main Methods:
- A nylon insert was designed for use with a reorienting gradient rotor.
- Infected plant tissues (fresh, frozen-thawed, mechanically disintegrated) were processed using the device and centrifugation.
- Virus extraction efficiency and purity of the concentrate were assessed.
Main Results:
- The nylon insert facilitated the separation of virus particles via centrifugal force.
- The device achieved an extraction yield of 80-90% of the fluid present in untreated plant tissue.
- Electron microscopy confirmed a significant reduction in extraneous materials in the virus concentrates.
Conclusions:
- The described nylon insert is an effective tool for plant virus extraction.
- The method provides high yields and purity, aiding in subsequent virus analysis.
- This device offers an improved approach for plant virus isolation in research and diagnostics.